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Differential transcript accumulation in Cicer arietinum L. in response to a chewing Insect Helicoverpa armigera and defense regulators correlate with reduced insect performance

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Title Differential transcript accumulation in Cicer arietinum L. in response to a chewing Insect Helicoverpa armigera and defense regulators correlate with reduced insect performance
 
Creator Singh, Archana
Singh, Indrakant Kumar
Verma, Praveen K.
 
Subject Chickpea
ET
Helicoverpa
induced plant defence
MeJA
SA
SSH
 
Description Monitoring transcriptional reorganization triggered in
response to a particular stress is an essential first step
for the functional analysis of genes involved in the
process. To characterize Cicer arietinum L. defence
responses against Helicoverpa armigera feeding, transcript patterns elicited by both herbivore and mechanical wounding were profiled and compared, and the
application of defence regulators was assessed. A
combination of approaches was employed to develop
transcript profiles, including suppression subtractive
hybridization (SSH), macroarray, northern blot, and
cluster analysis. Of the 63 unique genes isolated,
29 genes expressed differentially when Helicoverpa
feeding and wounding responses were compared.
Comparative macroarray analyses revealed that most
of the Helicoverpa-induced transcripts were methyl
jasmonate (MeJA) and ethylene (ET) regulated. The
effects of mild insect infestation and the exogenous
application of signalling compounds on larval feeding
behaviour were also monitored. Bioassays were performed to measure dispersal percentage and growth of
larvae on elicited plants. Larvae released on elicited
plants had decreased larval performance, demonstrating the central role of induced plant defence against
herbivory. Similarly, wounding and exogenous application of MeJA and ET also affected larval growth
and feeding behaviour. Our results demonstrated that
Helicoverpa attack up-regulated large transcriptional
changes and induced chickpea defence responses. Therefore, the results of this study advance the understanding of non-model plant–insect interactions on
a broader scale.
This research was supported by the National Institute of Plant
Genome Research, New Delhi.
 
Date 2013-11-12T06:29:53Z
2013-11-12T06:29:53Z
2008
17 March 2008
 
Type Article
 
Identifier J. Exp. Bot., 59(9): 2379-2392
http://hdl.handle.net/123456789/102
 
Language en
 
Publisher Oxford University Press