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Predominance of the serpentine route in monoterpenoid indole alkaloid pathway of Catharanthus roseus

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Title Predominance of the serpentine route in monoterpenoid indole alkaloid pathway of Catharanthus roseus
 
Creator Singh, Digvijay
Rai, Sanjay Kumar
Pandey-Rai, Shashi
Srivastava, Suchi
Mishra, Raghvendra Kumar
Sharma, Srikant
Kumar, Sushil
 
Subject Catharanthus roseus
Medicinal alkaloids of Catharanthus roseus
Terpenoid indole alkaloids
Organ-wise alkaloid compartmentation
Serpentine, catharanthine and vindoline biosynthetic routes
Serpentine route predominance
 
Description Single runs of RP-HPLC procedure were used for simultaneous and organ-wise quantification of the terpenoid indole
alkaloids (TIAs) serpentine, ajmalicine, vindoline, catharanthine, vincristine and vinblastine in whole adult plants of
Catharanthus roseus, sampled from 81 accessions and F2 generation of a cross. The average total TIA content was 159 mg
distributed among stem, root, leaf and inflorescence organs in 13:8:3:1 proportion. The root and stem barks, leaf-petiole
and corolla petals were several fold TIA-richer than root and stem woods, leaf–lamina and rest of flower organs, respectively. TIAs were undetectable in seed but present in the seedling. Quantitatively, serpentine (+ ajmalicine), serpentine and
catharanthine, vindoline, serpentine, catharanthine and vinblastine (+ vincristine) were the principal TIAs detected in
stem, root, leaf and inflorescence organs, respectively. Significant amounts of vinblastine, vindoline and catharanthine
were found present in the non-chlorophyllous corolla petals. Correlation studies showed that contents of catharanthine
and serpentine in roots, catharanthine, vindoline and vinblastine in leaves, serpentine in roots and stems and stems and
leaves were interdependent. Serpentine could be involved in suppressing the accumulation of other TIAs in leaves. The
observed inter- and intra- organ contents of end-products from serpentine, catharanthine and vindoline TIA branch pathways and correlations between them showed predominance of the serpentine branch.
The work was
financially supported by the Department of
Biotechnology, Council of Scientific and Industrial
Research (CSIR) and National Institute for Plant Genome
Research (NIPGR), Government of India.
 
Date 2013-11-12T06:39:39Z
2013-11-12T06:39:39Z
2008
30 November 2008
 
Type Article
 
Identifier Proc. Ind. Natl. Sci. Acad., 74(3): 97-109
http://hdl.handle.net/123456789/103
 
Language en
 
Publisher Indian National Science Academy