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Fine mapping of loci involved with glucosinolate biosynthesis in oilseed mustard (Brassica juncea) using genomic information from allied species

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Title Fine mapping of loci involved with glucosinolate biosynthesis in oilseed mustard (Brassica juncea) using genomic information from allied species
 
Creator Bisht, Naveen C.
Gupta, V.
Ramchiary, N.
Sodhi, Y. S.
Mukhopadhyay, A.
Arumugam, N.
Pental, D.
Pradhan, A. K.
 
Subject Brassica juncea
oilseed mustard
Fine mapping of loci
glucosinolate biosynthesis
 
Description Fine mapping of six seed glucosinolate QTL
(J2Gsl1, J3Gsl2, J9Gsl3, J16Gsl4, J17Gsl5 and J3Gsl6)
(Ramchiary et al. in Theor Appl Genet 116:77–85, 2007a)
was undertaken by the candidate gene approach. Based on
the DNA sequences from Arabidopsis and Brassica oleracea for the diVerent genes involved in the aliphatic glucosinolate biosynthesis, candidate genes were ampliWed and
sequenced from high to low glucosinolate Brassica juncea lines Varuna and Heera, respectively. Of the 20 paralogues
identiWed, 17 paralogues belonging to six gene families
were mapped to 12 of the 18 linkage groups of B. juncea
genome. Co-mapping of candidate genes with glucosinolate
QTL revealed that the candidate gene BjuA.GSL-ELONG.a
mapped to the QTL interval of J2Gsl1, BjuA.GSL-
ELONG.c, BjuA.GSL-ELONG.d and BjuA.Myb28.a
mapped to the QTL interval of J3Gsl2, BjuA.GSL-ALK.a
mapped to the QTL interval of J3Gsl6 and BjuB.Myb28.a
mapped to the QTL interval of J17Gsl5. The QTL J9Gsl3
and J16Gsl4 did not correspond to any of the mapped candidate genes. The functionality and contribution of diVerent
candidate genes/QTL was assessed by allelic variation
study using phenotypic data of 785 BC4DH lines. It was
observed that BjuA.Myb28.a and J9Gsl3 contributed significantly to the base level glucosinolate production while
J16Gsl4, probably GSL-PRO, BjuA.GSL-ELONG.a and
BjuA.GSL-ELONG.c contributed to the C3, C4 and C5
elongation pathways, respectively. Three A genome QTL:
J2Gsl1harbouring BjuA.GSL-ELONG.a, J3Gsl2 harbouring
both BjuA.GSL-ELONG.c and BjuA.Myb28.a and J9Gsl3,
possibly the ‘Bronowski genes’, were identiWed as most
important loci for breeding low glucosinolate B. juncea.
We observed two-step genetic control of seed glucosinolate
in B. juncea mainly eVected by these three A genome QTL.
This study, therefore, provides clues to the genetic mechanism of ‘Bronowski genes’ controlling the glucosinolate
trait and also provides eYcient markers for marker-assisted
introgression of low glucosinolate trait in B. juncea.
This work was supported by the Dhara Vegetable Oil and Food Company Ltd (DOFCO), a fully owned company of
the National Dairy Development Board (NDDB) and the Department
of Biotechnology (DBT). Partial support came from UGC-SAP programme.
 
Date 2013-11-12T10:06:09Z
2013-11-12T10:06:09Z
2009
27 September 2008
 
Type Article
 
Identifier Theor. Appl. Genet., 118: 413-421
http://hdl.handle.net/123456789/105
 
Language en
 
Publisher Springer