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Differential responses of arsenic stress in two varieties of Brassica juncea

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Title Differential responses of arsenic stress in two varieties of Brassica juncea
 
Creator Gupta, Meetu
Sharma, Pallavi
Sarin, Neera Bhalla
Sinha, Alok Krishna
 
Subject Arsenite
Detoxification
Phytochelatin synthase
Antioxidant enzymes
MAP kinase
Stress
 
Description Present study showed the toxicity caused by Arsenite (As(III)) and its detoxification responses in two varieties (Varuna and Pusa Bold) of Brassica juncea. Comparisons were made in leaves and roots of both the varieties, which showed that the accumulation pattern in both the varieties were dose and duration dependent, being more in roots for two days and in leaves for four days. Increase/decrease of antioxidant enzymes activities (SOD, CAT, GPX) showed not much changes at the given concentrations except that the enzyme activities showed significant increase at the lower concentrations. Semi quantitative RT-PCR analysis of PCS showed more expression of its transcript in P. Bold as compared to Varuna variety due to As(III) stress. The analysis of isoenzyme pattern in leaves of P. Bold showed five and two major bands of SOD and GPX, respectively. As(III) treatment leads to the activation of MAPK activity indicating role of this important cascade in transducing As(III) mediated signals. The data presented indicates the differential responses in both the varieties and also that the increased tolerance in P. Bold may be due to the defensive role of antioxidant enzymes, induction of MAPK and up regulation of PCS transcript which is responsible for the production of metal binding peptides.
M.G. acknowledges financial support from Council of Scientific
and Industrial Research (CSIR), New Delhi, India.
 
Date 2013-11-20T06:23:43Z
2013-11-20T06:23:43Z
2009
12 November 2008
 
Type Article
 
Identifier Chemosphere, 74: 1201-1208
http://hdl.handle.net/123456789/111
 
Language en
 
Publisher Elsevier B.V.