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Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice

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Title Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice
 
Creator Jain, Mukesh
Khurana, Jitendra P.
 
Subject abiotic stress
auxin
microarray analysis
reproductive development
rice (Oryza sativa)
 
Description Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Arabidopsis in detail, but not in crop plants. Earlier, we reported the identification and characterization of the members of the GH3, Aux/IAA and SAUR gene families in rice. In this study, whole genome microarray analysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A comparison of expression profiles of untreated and auxin-treated rice seedlings identified 315 probe sets representing 298 (225 upregulated and 73 downregulated) unique genes as auxin-responsive. Functional categorization revealed that genes involved in various biological processes, including metabolism, transcription, signal transduction, and transport, are regulated by auxin. The expression profiles of auxin-responsive genes identified in this study and those of the members of the GH3, Aux/IAA, SAUR and ARF gene families were analyzed during various stages of vegetative and reproductive (panicle and seed) development by employing microarray analysis. Many of these genes are, indeed, expressed in a tissue-specific or developmental stage-specific manner, and the expression profiles of some of the representative genes were confirmed by real-time PCR. The differential expression of auxin-responsive genes during various stages of panicle and seed development implies their involvement in diverse developmental processes. Moreover, several auxin-responsive genes were differentially expressed under various abiotic stress conditions, indicating crosstalk between auxin and abiotic stress signaling.
M. Jain acknowledges financial support from the Department of Biotechnology, Government of India, New Delhi, under the Innovative Young Biotechnologists Award scheme, and a core grant from NIPGR.
 
Date 2013-11-22T07:11:47Z
2013-11-22T07:11:47Z
2009
31 March 2009
 
Type Article
 
Identifier FEBS Journal, 276(11): 3148-3162
http://hdl.handle.net/123456789/116
 
Language en
 
Publisher Wiley-Blackwell