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Mapping of the multifoliate pinna (mfp) leaf-blade morphology mutation in grain pea Pisum sativum

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Title Mapping of the multifoliate pinna (mfp) leaf-blade morphology mutation in grain pea Pisum sativum
 
Creator Mishra, Raghvendra Kumar
Kumar, Anil
Chaudhary, Swati
Kumar, Sushil
 
Subject Pisum sativum
multifoliate pinna
grain pea
leaf-blade morphology mutation
 
Description The multifoliate pinna (mfp) mutation alters the leaf-blade architecture of pea, such that simple tendril pinnae of distal domain are replaced by compound pinna blades of tendrilled leaflets in mfp homozygotes. The MFP locus was mapped with reference to DNA markers using F2 and F2:5 RIL as mapping populations. Among 205 RAPD, 27 ISSR and 35 SSR markers that demonstrated polymorphism between the parents of mapping populations, three RAPD markers were found linked to the MFP locus by bulk segregant analyses on mfp/mfp and MFP/MFP bulks assembled from the F2:5 population. The segregational analysis of mfp and 267 DNA markers on 96 F2 plants allowed placement of 26 DNA markers with reference to MFP on a linkage group. The existence of common markers on reference genetic maps and MFP linkage group developed here showed that MFP is located on linkage group IV of the consensus genetic map of pea.
Grateful thanks are due to the Director, NIPGR for providing facilities and to the Indian National Science Academy for grant of
INSA Senior Scientist Scheme to SK.
 
Date 2014-01-07T11:04:39Z
2014-01-07T11:04:39Z
2009
30 March 2009
 
Type Article
 
Identifier J. Genet., 88(2): 227-232
http://hdl.handle.net/123456789/121
 
Language en
 
Publisher Indian Academy of Sciences