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CAP2 enhances germination of transgenic tobacco seeds at high temperature and promotes heat stress tolerance in yeast

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Title CAP2 enhances germination of transgenic tobacco seeds at high temperature and promotes heat stress tolerance in yeast
 
Creator Shukla, Rakesh Kumar
Tripathi, Vineeta
Jain, Deepti
Yadav, Rajiv Kumar
Chattopadhyay, Debasis
 
Subject CAP2
chickpea
heat stress
tobacco
yeast
 
Description We reported earlier that ectopic expression of CAP2, a single AP2 domain
containing transcription activator from chickpea (Cicer arietinum) in
tobacco improves growth and development, and tolerance to dehydration
and salt stress, of the transgenic plants. Here, we report that, in addition,
the CAP2-transgenic tobacco seeds also exhibit higher germination efficiency at high temperature and show higher expression levels of genes for
tobacco heat shock proteins and a heat shock factor. CAP2 was able to
activate the 5¢-upstream activating sequence of tobacco heat shock factor.
Surprisingly, expression of CAP2 cDNA in Saccharomyces cerevisiae also
enhanced heat tolerance, with increased expression of the gene for yeast
heat shock factor 1 (Hsf1) and its target, the gene for yeast heat shock protein 104 (Hsp104). Sequence analysis of the Hsf1 promoter revealed the
presence of a dehydration-responsive element ⁄ C-repeat-like element (DRE/
CRE). Recombinant CAP2 protein bound to the DRE/CRE in the Hsf1
promoter in a gel shift assay and transactivated the Hsf1 promoter–His
reporter construct. The full-length CAP2 protein was required to provide
thermotolerance in yeast. If these findings are taken together, our results
suggest that CAP2 is involved in the heat stress response and provides an
example of functioning of a plant transcription factor in yeast, highlighting
the strong evolutionary conservation of the stress response mechanism.
 
Date 2014-02-13T09:54:06Z
2014-02-13T09:54:06Z
2009
16 July 2009
 
Type Article
 
Identifier FEBS J., 276: 5252-5262
http://hdl.handle.net/123456789/129
 
Language en
 
Publisher Wiley-Blackwell