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CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plant

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Title CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plant
 
Creator Tripathi, Vineeta
Parasuraman, Boominathan
Laxmi, Ashverya
Chattopadhyay, Debasis
 
Subject CIPK6
Arabidopsis
chickpea
auxin
root
Stress
 
Description Calcineurin B-like proteins (CBL) and CBL-interacting protein kinases (CIPK) mediate plant responses to a
variety of external stresses. Here we report that Arabidopsis CIPK6 is also required for the growth and
development of plants. Phenotype of tobacco plants ectopically expressing a homologous gene (CaCIPK6)
from the leguminous plant chickpea (Cicer arietinum) indicated its functional conservation. A lesion in AtCIPK6
significantly reduced shoot-to-root and root basipetal auxin transport, and the plants exhibited developmental
defects such as fused cotyledons, swollen hypocotyls and compromised lateral root formation, in conjunction
with reduced expression of a number of genes involved in auxin transport and abiotic stress response. The
Arabidopsis mutant was more sensitive to salt stress compared to wild-type, while overexpression of a
constitutively active mutant of CaCIPK6 promoted salt tolerance in transgenic tobacco. Furthermore, tobacco
seedlings expressing the constitutively active mutant of CaCIPK6 showed a developed root system, increased
basipetal auxin transport and hypersensitivity to auxin. Our results provide evidence for involvement of a CIPK
in auxin transport and consequently in root development, as well as in the salt-stress response, by regulating
the expression of genes.
The project was funded by the Department of Biotechnology,
Government of India, and by a seed grant from the National Institute
for Plant Genome Research. V.T. acknowledges support from the
Council for Scientific and Industrial Research, and B.P. acknowledges research fellowships from the University Grants Commission.
 
Date 2014-02-13T10:15:22Z
2014-02-13T10:15:22Z
2009
19 January 2009
 
Type Article
 
Identifier Plant Journal, 58: 778-790
http://hdl.handle.net/123456789/131
 
Language en
 
Publisher Wiley-Blackwell