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Soybean cultivar resistant to mungbean yellow mosaic India virus infection induces viral RNA degradation earlier than the susceptible cultivar

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Title Soybean cultivar resistant to mungbean yellow mosaic India virus infection induces viral RNA degradation earlier than the susceptible cultivar
 
Creator Yadav, Rajiv Kumar
Shukla, Rakesh Kumar
Chattopadhyay, Debasis
 
Subject Begomovirus
Mungbean Yellow Mosaic India Virus
Soybean
Agroinoculation
Post-transcriptional gene silencing
 
Description Yellow mosaic disease caused by whitefly-transmitted bipartite Geminiviruses is one of the major con-
straints on productivity of a number of pulse crops. We have cloned the bipartite genome of Mungbean
Yellow Mosaic India Virus isolated from infected Soybean. We report here that agroinfection of Soybean
seedlings with a single uncut recombinant binary plasmid containing tandem dimers of both DNA A and
DNA B resulted in 100% infectivity in susceptible varieties. To understand the mechanism of natural resistance in a Soybean variety, we compared the abundance of the viral RNAs in a resistant and a susceptible
variety at the early time points after agroinfection. Whilst the resistant variety displayed synthesis but
rapid degradation of the early viral RNAs; the degradation in the susceptible variety was delayed resulting
in accumulation of those transcripts later in infection. Accumulation of the late viral transcripts and DNA
replication were detectable only in the susceptible variety. This indicates that rapid degradation of the
early viral transcripts, possibly through siRNA mechanism, is one of the probable mechanisms of natural
resistance against geminivirus.
This work is funded by a seed grant from the National Institute
of Plant Genome Research. RKY acknowledges Council for Scientific
and Industrial Research, India for fellowship.
 
Date 2014-02-13T10:43:26Z
2014-02-13T10:43:26Z
2009
5 April 2009
 
Type Article
 
Identifier Virus Res., 144: 89-95
http://hdl.handle.net/123456789/135
 
Language en
 
Publisher Elsevier B.V.