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Characterization of a pollen-preferential gene OSIAGP from rice (Oryza sativa L. subspecies indica) coding for an arabinogalactan-protein homolog and analysis of its promoter activity during pollen development and pollen tube growth

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Title Characterization of a pollen-preferential gene OSIAGP from rice (Oryza sativa L. subspecies indica) coding for an arabinogalactan-protein homolog and analysis of its promoter activity during pollen development and pollen tube growth
 
Creator Anand, Saurabh
Tyagi, Akhilesh K.
 
Subject Arabinogalactan protein
Pollen-preferential gene
Pollen tube
Rice
Tobacco
Transgenic
 
Description During differential screening of inflorescence-specific cDNA libraries from Oryza sativa indica, an arabinogalactan protein (OSIAGP) cDNA (586 bp) expressing preferentially in the inflorescence has been isolated. It encodes an arabinogalactan protein of 59 amino acids (6.4 kDa) with a transmembrane domain and a secretory domain at the N terminus. The protein shows homology with AGP23 from Arabidopsis, and its homologue in japonica rice is located on chromosome 6. OSIAGP transcripts also accumulate in shoots and roots of rice seedling grown in the dark, but light represses expression of the gene. Analysis of a genomic clone of OSIAGP revealed that its promoter contains several pollen-specificity and light-regulatory elements. The promoter confers pollen-preferential activity on gus, starting from the release of microspores to anther dehiscence in transgenic tobacco, and is also active during pollen tube growth. Analysis of pollen preferential activity of the promoter in the transgenic rice system revealed that even the approximately 300 bp fragment has activity in pollen and the anther wall and further deletion down to approximately 100 bp completely abolishes this activity, which is consistent with in-silico analysis of the promoter. Arabinogalactan proteins have been shown to be involved in the cell elongation process. The homology of OSIAGP with AGP23 and the fact that seedling growth in the dark and pollen tube growth are events based on cell elongation strengthen the possibility of OSIAGP performing a similar function.
The research was supported by a grant
from the Department of Biotechnology, Government of India.
SA was a recipient of JRF and SRF given by University Grants
Commission.
 
Date 2014-02-20T04:48:06Z
2014-02-20T04:48:06Z
2010
19 August 2009
 
Type Article
 
Identifier Transgenic Res., 19(3): 385-397
http://hdl.handle.net/123456789/136
 
Language en
 
Publisher Springer