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Variability in Indian bread wheat (Triticum aestivum L.) varieties differing in nitrogen efficiency as assessed by microsatellite markers

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Title Variability in Indian bread wheat (Triticum aestivum L.) varieties differing in nitrogen efficiency as assessed by microsatellite markers
 
Creator Chandna, Ruby
Gupta, Sarika
Ahmad, Altaf
Iqbal, Muhammad
Prasad, Manoj
 
Subject Bread wheat
Triticum aestivum
Nitrogen efficiency
Genetic similarity
Microsatellite/SSR markers
 
Description Wheat (Triticum aestivum L.) is a staple food for half of the world. Its productivity and agronomical practices, especially for nitrogen supplementation, is governed by the nitrogen efficiency (NE) of the genotypes. We analyzed 16 popular cultivated Indian varieties of wheat for their NE and variability estimates using a set of 21 simple sequence repeat (SSR) markers, derived from each wheat chromosome. These genotypes were categorized into three groups, viz., low, moderate, and high nitrogen efficient. Of these 16 genotypes, we have reported six, eight, and two genotypes in high, moderate, and low NE categories, respectively. The differential NE in these genotypes was supported by nitrogen uptake and assimilation parameters. The values of average polymorphic information content and marker index for these SSR markers were estimated to be 0.32 and 0.59, respectively. The genetic similarity coefficient for all possible pairs of varieties ranged from 0.41 to 0.76, indicating the presence of considerable range of genetic diversity at molecular level. The dendrogram prepared on the basis of unweighted pair-group method of arithmetic average algorithm grouped the 16 wheat varieties into three major clusters. The clustering was strongly supported by high bootstrap values. The distribution of the varieties in different clusters and subclusters appeared to be related to their variability in NE parameter that was scored. Genetically diverse parents were identified that could potentially be used for their desirable characteristics in breeding programs for improvement of NE in wheat.
We are thankful to the Director of the Indian
Agricultural Research Institute, Pusa, New Delhi for providing the
seed material; to Drs. M. Röder and M. Ganal, IPK, Gatersleben,
Germany for providing DNA aliquots of some of the unpublished SSR
primers and Dr. Pankaj Kaushal for helpful discussions. Grateful
thanks are also due to the Director of the National Institute of Plant
Genome Research, and Head, Department of Botany, Jamia Hamdard,
New Delhi, India for providing facilities. We also gratefully
acknowledge the financial support from the Department of Biotechnology, Department of Science and Technology, and University Grants
Commission, Government of India for carrying out the present study.
 
Date 2014-02-20T09:20:27Z
2014-02-20T09:20:27Z
2010
15 February 2010
 
Type Article
 
Identifier Protoplasma, 242(1-4): 55-67
http://hdl.handle.net/123456789/141
 
Language en
 
Publisher Springer