Record Details

Validation of internal control genes for quantitative gene expression studies in chickpea (Cicer arietinum L.)

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Validation of internal control genes for quantitative gene expression studies in chickpea (Cicer arietinum L.)
 
Creator Garg, Rohini
Sahoo, Annapurna
Tyagi, Akhilesh K.
Jain, Mukesh
 
Subject Chickpea (Cicer arietinum)
Gene expression
Housekeeping genes
Internal control
Normalization
Real-time PCR
 
Description The real-time polymerase chain reaction (PCR) data requires normalization with an internal control gene expressed at constant levels under all the experimental conditions being analyzed for accurate and reliable gene expression results. In this study, the expression of 12 candidate internal control genes, including ACT1, EF1alpha, GAPDH, IF4a, TUB6, UBC, UBQ5, UBQ10, 18SrRNA, 25SrRNA, GRX and HSP90, in a diverse set of 18 tissue samples representing different organs/developmental stages and stress conditions in chickpea (Cicer arietinum L.) has been validated. Their expression levels vary considerably in various tissue samples analyzed. The expression levels of EF1alpha and HSP90 are most constant across various organs/developmental stages analyzed. Similarly, the expression levels of IF4a and GAPDH are most constant across various stress conditions. A set of two most stable genes is found sufficient for accurate and reliable normalization of real-time PCR data in the given set of tissue samples of chickpea. The genes with most constant expression identified in this study should be useful for normalization of gene expression data in a wide variety of tissue samples in chickpea.
This study was supported financially by the Department of Biotechnology, Government of India, New Delhi, under the Next Generation Challenge Programme on Chickpea Genomics and core grant from the NIPGR.
 
Date 2014-02-20T09:57:19Z
2014-02-20T09:57:19Z
2010
April 2010
 
Type Article
 
Identifier Biochem. Biophys. Res. Comm., 396(2): 283-288
http://hdl.handle.net/123456789/145
 
Language en
 
Publisher Elsevier B.V.