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The water-deficit stress and red-rot-related genes in sugarcane

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Title The water-deficit stress and red-rot-related genes in sugarcane
 
Creator Gupta, Vikrant
Raghuvanshi, Saurabh
Gupta, Ambika
Saini, Navin
Gaur, Anupama
Khan, M.S.
Gupta, R.S.
Singh, J.
Duttamajumder, S.K.
Srivastava, S.
Suman, A.
Khurana, Jitendra P.
Kapur, Raman
Tyagi, Akhilesh K.
 
Subject cDNA library
Disease-related genes
EST
Quantitative real-time PCR
Red-rot
Water-deficit stress-related genes
Sugarcane
 
Description Sugarcane is an important international commodity as a valuable agricultural crop especially in developing countries. Sequencing was carried out to generate >35,000 expressed sequence tags (ESTs) from healthy as well as red-rot-infected tissue of Indian subtropical variety of sugarcane. Subsequent clustering with existing sugarcane ESTs in public databases identified 4,087 clusters, including 85 clusters that preferentially express upon Colletotrichum falcatum (red-rot) infection, which were previously unreported. Real-time reverse transcription-PCR profiling of selected EST clusters identified several sugarcane clusters that show differential expression in response to biotic and abiotic stress conditions. Twenty-five stress-related clusters showed >2-fold relative expression during water-deficit stress in sugarcane. Similarly, EST clusters could be identified, which exhibit association with red-rot disease when assessed in red-rot-susceptible and red-rot-resistant varieties of sugarcane. Such EST clusters are good candidates for in-depth analysis to elucidate stress-responsive pathways in sugarcane and facilitate genetic manipulation to tailor this crop for tolerance to various stresses.
We gratefully acknowledge the financial support of Department of Biotechnology and University Grants Commission, Government of India.
 
Date 2014-02-20T11:03:10Z
2014-02-20T11:03:10Z
2010
16 September 2009
 
Type Article
 
Identifier Funct. Integr. Genomics, 10(2): 207-214
http://hdl.handle.net/123456789/146
 
Language en
 
Publisher Springer