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Genetic control of leaf-blade morphogenesis by the Insecatus gene in Pisum sativum

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Title Genetic control of leaf-blade morphogenesis by the Insecatus gene in Pisum sativum
 
Creator Kumar, Sushil
Chaudhary, Swati
Sharma, Vishakha
Kumari, Renu
Mishra, Raghvendra Kumar
Kumar, Arvind
Choudhury, Debjani Roy
Jha, Ruchi
Priyadarshini, Anupama
Kumar, Arun
 
Subject Pisum sativum
Insecatus gene
leaf-blade morphogenesis
 
Description To understand the role of INSECATUS (INS) gene in pea, the leaf blades of wild-type, ins mutant and seven other genotypes, constructed by recombining ins with uni-tac, af, tl and mfp gene mutations, were quantitatively compared. The ins was inherited as a recessive mutant allele and expressed its phenotype in proximal leaflets of full size leaf blades. In ins leaflets, the midvein development was arrested in distal domain and a cleft was formed in lamina above this point. There was change in the identity of ins leaflets such that the intercalary interrupted midvein bore a leaf blade. Such adventitious blades in ins, ins tl and ins tl mfp were like the distal segment of respective main leaf blade. The ins phenotype was not seen in ins af and ins af uni-tac genotypes. There was epistasis of uni-tac over ins. The ins, tl and mfp mutations interacted synergistically to produce highly pronounced ins phenotype in the ins tl mfp triple mutant. The role(s) of INS in leaf-blade organogenesis are: positive regulation of vascular patterning in leaflets, repression of UNI activity in leaflet primordia for ectopic growth and in leaf-blade primordium for indeterminate growth of rachis, delimitation of proximal leaflet domain and together with TL and MFP homeostasis for meristematic activity in leaflet primordia. The variant apically bifid shape of the affected ins leaflets demonstrated that the leaflet shape is dependent on the venation pattern.
Grateful thanks are due to the Director, NIPGR, for facilities and to
Indian National Science Academy for the grant of Senior Scientist
Scheme and to Council of Scientific and Industrial Research for the
grant of emeritus scientistship to SK.
 
Date 2014-02-20T11:33:37Z
2014-02-20T11:33:37Z
2010
29 March 2010
 
Type Article
 
Identifier J. Genet., 89(2): 201-211
http://hdl.handle.net/123456789/149
 
Language en
 
Publisher Indian Academy of Sciences