Genetic control of leaf-blade morphogenesis by the Insecatus gene in Pisum sativum
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Title |
Genetic control of leaf-blade morphogenesis by the Insecatus gene in Pisum sativum
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Creator |
Kumar, Sushil
Chaudhary, Swati Sharma, Vishakha Kumari, Renu Mishra, Raghvendra Kumar Kumar, Arvind Choudhury, Debjani Roy Jha, Ruchi Priyadarshini, Anupama Kumar, Arun |
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Subject |
Pisum sativum
Insecatus gene leaf-blade morphogenesis |
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Description |
To understand the role of INSECATUS (INS) gene in pea, the leaf blades of wild-type, ins mutant and seven other genotypes, constructed by recombining ins with uni-tac, af, tl and mfp gene mutations, were quantitatively compared. The ins was inherited as a recessive mutant allele and expressed its phenotype in proximal leaflets of full size leaf blades. In ins leaflets, the midvein development was arrested in distal domain and a cleft was formed in lamina above this point. There was change in the identity of ins leaflets such that the intercalary interrupted midvein bore a leaf blade. Such adventitious blades in ins, ins tl and ins tl mfp were like the distal segment of respective main leaf blade. The ins phenotype was not seen in ins af and ins af uni-tac genotypes. There was epistasis of uni-tac over ins. The ins, tl and mfp mutations interacted synergistically to produce highly pronounced ins phenotype in the ins tl mfp triple mutant. The role(s) of INS in leaf-blade organogenesis are: positive regulation of vascular patterning in leaflets, repression of UNI activity in leaflet primordia for ectopic growth and in leaf-blade primordium for indeterminate growth of rachis, delimitation of proximal leaflet domain and together with TL and MFP homeostasis for meristematic activity in leaflet primordia. The variant apically bifid shape of the affected ins leaflets demonstrated that the leaflet shape is dependent on the venation pattern.
Grateful thanks are due to the Director, NIPGR, for facilities and to Indian National Science Academy for the grant of Senior Scientist Scheme and to Council of Scientific and Industrial Research for the grant of emeritus scientistship to SK. |
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Date |
2014-02-20T11:33:37Z
2014-02-20T11:33:37Z 2010 29 March 2010 |
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Type |
Article
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Identifier |
J. Genet., 89(2): 201-211
http://hdl.handle.net/123456789/149 |
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Language |
en
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Publisher |
Indian Academy of Sciences
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