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Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress

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Title Comparative transcriptome analysis of differentially expressed genes in foxtail millet (Setaria italica L.) during dehydration stress
 
Creator Lata, Charu
Sahu, Pranav Pankaj
Prasad, Manoj
 
Subject Setaria italica
Dehydration stress
Relative Water Content (RWC)
Suppression Subtractive Hybridization (SSH)
Reverse Northern (RN)
Quantitative real time PCR (qRT-PCR)
Gene expression
 
Description hydration stress is one of the most important abiotic stresses that adversely influence crop growth and productivity. With the aim to understand the molecular mechanisms underlying dehydration stress tolerance in foxtail millet (Setaria italica L.), a drought tolerant crop, we examined its transcriptome changes at two time points (early and late) of dehydration stress. Two suppression subtractive hybridization (SSH) forward libraries were constructed from 21-day old seedlings of tolerant cv. Prasad at 0.5 and 6h PEG-induced dehydration stress. A total of 327 unique ESTs were identified from both libraries and were classified into 11 different categories according to their putative functions. The plant response against dehydration stress was complex, representing major transcripts involved in metabolism, stress, signaling, transcription regulation, translation and proteolysis. By Reverse Northern (RN) technique we identified the differential expression pattern of 327 transcripts, 86 (about 26%) of which showed > or = 1.7-fold induction. Further the obtained results were validated by quantitative real-time PCR (qRT-PCR) to have a comparative expression profiling of randomly chosen 9 up-regulated transcripts (> or =2.5 fold induction) between cv. Prasad (tolerant) and cv. Lepakshi (sensitive) upon dehydration stress. These transcripts showed a differential expression pattern in both cultivars at different time points of stress treatment as analyzed by qRT-PCR. The possible relationship of the identified transcripts with dehydration tolerance mechanism is discussed.
Grateful thanks are due to UGC, Government of India for providing UGC-JRF to Ms Charu Lata and to DBT, Government of India for
providing financial support.
 
Date 2014-02-21T05:12:02Z
2014-02-21T05:12:02Z
2010
18 February 2010
 
Type Article
 
Identifier Biochem. Biophys. Res. Comm., 393(4):720-727
http://hdl.handle.net/123456789/151
 
Language en
 
Publisher Elsevier B.V.