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Tomato cultivar tolerant to tomato leaf curl New Delhi Virus infection induces virus-specific siRNA accumulation and defense associated host gene expression

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Title Tomato cultivar tolerant to tomato leaf curl New Delhi Virus infection induces virus-specific siRNA accumulation and defense associated host gene expression
 
Creator Sahu, Pranav Pankaj
Rai, Neeraj K.
Chakraborty, Supriya
Singh, Major
Chandrappa, Prasanna H.
Ramesh, Bandarupalli
Chattopadhyay, Debasis
Prasad, Manoj
 
Subject New Delhi virus
Tomato cultivar tolerant to Tomato leaf curl
RNA accumulation
Gene expression
 
Description Tomato leaf curl New Delhi virus (ToLCNDV) infection causes significant yield loss in tomato. The availability of a conventional tolerance source against this virus is limited in tomato. To understand the molecular mechanism of virus tolerance in tomato, the abundance of viral genomic replicative intermediate molecules and virus-directed short interfering RNAs (siRNAs) by the host plant in a naturally tolerant cultivar H-88-78-1 and a susceptible cultivar Punjab Chhuhara at different time points after agroinfection was studied. We report that less abundance of viral replicative intermediate in the tolerant cultivar may have a correlation with a relatively higher accumulation of virus-specific siRNAs. To study defence-related host gene expression in response to ToLCNDV infection, the suppression subtractive hybridization technique was used. A library was prepared from tolerant cultivar H-88-78-1 between ToLCNDV-inoculated and Agrobacterium mock-inoculated plants of this cultivar at 21 days post-inoculation (dpi). A total of 106 nonredundant transcripts was identified and classified into 12 different categories according to their putative functions. By reverse Northern analysis and quantitative real-time polymerase chain reaction (qRT-PCR), we identified the differential expression pattern of 106 transcripts, 34 of which were up-regulated (>2.5-fold induction). Of these, eight transcripts showed more than four fold induction. qRT-PCR analysis was carried out to obtain comparative expression profiling of these eight transcripts between Punjab Chhuhara and H-88-78-1 on ToLCNDV infection. The expression patterns of these transcripts showed a significant increase in differential expression in the tolerant cultivar, mostly at 14 and 21 dpi, in comparison with that in the susceptible cultivar, as analysed by qRT-PCR. The probable direct and indirect relationship of siRNA accumulation and up-regulated transcripts with the ToLCNDV tolerance mechanism is discussed.
We are grateful to the Department of Biotechnology, Government of India for
providing financial support (Grant no. BT/PR/5274/AGR/16/464/2004).
 
Date 2014-02-24T09:13:59Z
2014-02-24T09:13:59Z
2010
May 2010
 
Type Article
 
Identifier Mol. Plant Pathology, 11(4): 531-544
http://hdl.handle.net/123456789/158
 
Language en
 
Publisher Wiley-Blackwell