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Identification, phylogeny and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato

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Title Identification, phylogeny and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato
 
Creator Sharma, Manoj K.
Kumar, Rahul
Solanke, Amolkumar U.
Sharma, Rita
Tyagi, Akhilesh K.
Sharma, Arun K.
 
Subject Development
Ethylene
Ethylene response factor
Expression
Ripening
Stress
 
Description Ethylene responsive transcription factors have been shown to be intimately connected to plant development, defense responses and stress signaling pathways and in order to use them for plant improvement, we need to have better understanding of these proteins. In this study, 85 ERF genes have been identified from tomato using raw EST data in various public repositories. Phylogenetic analysis with tomato ERF domains revealed their distribution in all the groups, previously identified in model systems. MEME motif analysis resulted in identification of conserved domains, characteristic to member of each clade, in addition to ERF domain. Expression analysis during vegetative and reproductive stages of development using QPCR and tomato GeneChip arrays, revealed their tissue-specific/preferential accumulation. In total, 57 genes were found to be differentially expressed during temporal stages of tomato fruit development. The expression analysis of 23 ERF family genes representing each clade in response to seven abiotic stress treatments revealed their differential expression in response to more than one abiotic stress treatments. Results suggest that ERF genes play diverse roles in plant's life and comprehensive data generated will be helpful in conducting functional genomics studies to understand their precise role during plant development and stress response.
This work has been funded by Department of
Biotechnology, Government of India. RK and AUS acknowledge
Council for Scientific and Industrial Research (CSIR) for senior
research fellowships.
 
Date 2014-02-24T09:22:17Z
2014-02-24T09:22:17Z
2010
14 September 2010
 
Type Article
 
Identifier Mol. Genet. Genomics, 284(6): 455-475
http://hdl.handle.net/123456789/159
 
Language en
 
Publisher Springer