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Stress induced beta subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase

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Title Stress induced beta subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase
 
Creator Bhardwaj, Deepak
Sheikh, Arsheed Hussain
Sinha, Alok Krishna
Tuteja, Narendra
 
Subject ABA
G-proteins
G-beta
methyl jasmonate
mitogen activated protein kinase
MPK3
signal transduction
yeast-two-hybrid assay
 
Description We here report in Pisum sativum system a novel protein-protein interaction of β-subunit of heterotrimeric G-proteins (PsGβ) with a Mitogen activated protein kinase (PsMPK3) during cDNA library screening by yeast-two-hybrid assay. The transcript of these two genes also showed co-regulation under abscisic acid (ABA) and methyl jasmonate (MeJA) treatments. The protein-protein interaction was further validated by performing one-to-one interaction and β-galactosidase assay in yeast system. β-subunit of G-proteins from a heterologous system Oryzae sativa also showed interaction with PsMPK3. The interaction between PsGβ and PsMPK3 was further confirmed by in vitro protein-protein interaction. This suggested that MPK3 function as effector molecule for Gβ, which may helps in the regulation of stomatal functioning. These findings also provide an evidence for a possible cross-talk between MPK3 and G-protein-mediated signaling pathways in plants.
This work is partially supported by
Department of Biotechnology (DBT), and Department of Science
and Technology (DST), Government of India
 
Date 2014-02-27T09:36:13Z
2014-02-27T09:36:13Z
2011
27 January 2011
 
Type Article
 
Identifier Plant Signal. Behav., 6(2): 287-292
http://hdl.handle.net/123456789/168
 
Language en
 
Publisher Landes Bioscience