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Conventional and novel Gγ protein families constitute the heterotrimeric G-protein signaling network in soybean

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Title Conventional and novel Gγ protein families constitute the heterotrimeric G-protein signaling network in soybean
 
Creator Choudhury, Swarup Roy
Bisht, Naveen C.
Thompson, Rheannon
Todorov, Oleg
Pandey, Sona
 
Subject G-Protein Signaling Network
Soybean
Heterotrimeric
Heterotrimeric G-proteins
 
Description Heterotrimeric G-proteins comprised of Gα, Gβ and Gγ proteins are important signal transducers in all eukaryotes. The Gγ protein of the G-protein heterotrimer is crucial for its proper targeting at the plasma membrane and correct functioning. Gγ proteins are significantly smaller and more diverse than the Gα and Gβ proteins. In model plants Arabidopsis and rice that have a single Gα and Gβ protein, the presence of two canonical Gγ proteins provide some diversity to the possible heterotrimeric combinations. Our recent analysis of the latest version of the soybean genome has identified ten Gγ proteins which belong to three distinct families based on their C-termini. We amplified the full length cDNAs, analyzed their detailed expression profile by quantitative PCR, assessed their localization and performed yeast-based interaction analysis to evaluate interaction specificity with different Gβ proteins. Our results show that ten Gγ genes are retained in the soybean genome and have interesting expression profiles across different developmental stages. Six of the newly identified proteins belong to two plant-specific Gγ protein families. Yeast-based interaction analyses predict some degree of interaction specificity between different Gβ and Gγ proteins. This research thus identifies a highly diverse G-protein network from a plant species. Homologs of these novel proteins have been previously identified as QTLs for grain size and yield in rice.
NB was supported by a visiting scholar fellowship from National Institute for Plant Genome Research, India and RT was supported by an National Science Foundation-Research Experiences for Undergraduates grant, (0851838). This work was supported by a United States Department of Agriculture/Agriculture and Food Research Initiative grant to SP, (2010-65116-20454).
 
Date 2014-04-04T04:59:28Z
2014-04-04T04:59:28Z
2011
13 July 2011
 
Type Article
 
Identifier PLoS One, 6(8): e23361
http://hdl.handle.net/123456789/172
 
Language en
 
Publisher Public Library of Science