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Analysis of differentially expressed genes in leaf rust infected bread wheat involving seeding resistance gene Lr28

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Title Analysis of differentially expressed genes in leaf rust infected bread wheat involving seeding resistance gene Lr28
 
Creator Dhariwal, Raman
Vyas, Shailendra
Bhaganagare, Govindraj R.
Jha, Shailendra K.
Khurana, Jitendra P.
Tyagi, Akhilesh K.
Prabhu, Kumble V.
Balyan, Harindra S.
Gupta, Pushpendra K.
 
Subject cDNA-AFLP analysis
compatible interaction
defense
incompatible interaction
Puccinia triticina
qRT-PCR analysis
TDFs
 
Description Genome-wide transcriptome analysis of seedling resistance to leaf rust conferred by Lr28 gene in wheat (Triticum aestivum L.) was conducted to identify differentially expressed genes during incompatible interaction. A virulent leaf rust race 77–5 was used for inoculation of resistant (HD2329 + Lr28) and susceptible (HD2329 – Lr28) wheat NILs and cDNA-AFLP analyses was carried out. As many as 223 differential transcripts appeared following leaf rust inoculation; these included 122 transcripts that appeared exclusively in resistant NIL, whereas 39 transcripts appeared both in resistant and susceptible NILs. Sequence analyses of 37 transcripts, which appeared in the resistant NIL revealed that 15 transcripts had homology with genes involved in protein synthesis, signal transduction, transport, disease resistance and metabolism. The functions of remaining 22 transcripts could not be determined; these included six novel genes reported for the first time in wheat. Specific primers could be designed for 18 of the 37 transcripts, which included genes with putative and unknown functions. Quantitative real time PCR analysis was conducted using these 18 pairs of primers. A majority (13) of these transcripts appeared within 48 h reaching a peak value at 96 h in resistant NIL signifying their role in providing leaf rust resistance.
Thanks are due to Department of Biotechnology, Government of India, for
providing financial support to carry out this study.
 
Date 2014-04-07T06:41:05Z
2014-04-07T06:41:05Z
2011
24 March 2011
 
Type Article
 
Identifier Func. Plant Biol., 38: 479-492
http://hdl.handle.net/123456789/175
 
Language en
 
Publisher CSIRO Publishing