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The N-glycan processing enzymes {alpha}-mannosidase and {beta}-D-N-acetylhexosaminidase are involved in ripening-associated softening in the non-climacteric fruits of Capsicum

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Title The N-glycan processing enzymes {alpha}-mannosidase and {beta}-D-N-acetylhexosaminidase are involved in ripening-associated softening in the non-climacteric fruits of Capsicum
 
Creator Ghosh, Sumit
Meli, Vijaykumar S.
Kumar, Anil
Thakur, Archana
Chakraborty, Niranjan
Chakraborty, Subhra
Datta, Asis
 
Subject Capsicum
climacteric
fruit softening
N-glycans
non-climacteric
RNAi
alpha-mannosidase
beta-D-N-acetylhexosaminidase
 
Description Excessive softening of fruits during the ripening process leads to deterioration. This is of significant global importance as softening-mediated deterioration leads to huge postharvest losses. N-glycan processing enzymes are reported to play an important role during climacteric fruit softening: however, to date these enzymes have not been characterized in non-climacteric fruit. Two ripening-specific N-glycan processing enzymes, α-mannosidase (α-Man) and β-D-N-acetylhexosaminidase (β-Hex), have been identified and targeted to enhance the shelf life in non-climacteric fruits such as capsicum (Capsicum annuum). The purification, cloning, and functional characterization of α-Man and β-Hex from capsicum, which belong to glycosyl hydrolase (GH) families 38 and 20, respectively, are described here. α-Man and β-Hex are cell wall glycoproteins that are able to cleave terminal α-mannose and β-D-N-acetylglucosamine residues of N-glycans, respectively. α-Man and β-Hex transcripts as well as enzyme activity increase with the ripening and/or softening of capsicum. The function of α-Man and β-Hex in capsicum softening is investigated through RNA interference (RNAi) in fruits. α-Man and β-Hex RNAi fruits were approximately two times firmer compared with the control and fruit deterioration was delayed by approximately 7 d. It is shown that silencing of α-Man and β-Hex enhances fruit shelf life due to the reduced degradation of N-glycoproteins which resulted in delayed softening. Altogether, the results provide evidence for the involvement of N-glycan processing in non-climacteric fruit softening. In conclusion, genetic engineering of N-glycan processing can be a common strategy in both climacteric and non-climacteric species to reduce the post-harvest crop losses.
This work
was supported by the Department of Biotechnology, the
Ministry of Science and Technology, Government of India.
 
Date 2014-04-22T06:08:09Z
2014-04-22T06:08:09Z
2011
3 September 2010
 
Type Article
 
Identifier J. Exp. Bot., 62(2): 571-582.
http://hdl.handle.net/123456789/184
 
Language en
 
Publisher Oxford University Press