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Development and utilization of novel intron length polymorphic markers in foxtail millet [Setaria italica (L.) P. Beauv.]

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Title Development and utilization of novel intron length polymorphic markers in foxtail millet [Setaria italica (L.) P. Beauv.]
 
Creator Gupta, Sarika
Kumari, Kajal
Das, Jyotirmoy
Lata, Charu
Puranik, Swati
Prasad, Manoj
 
Subject Foxtail millet (Setaria italica L.)
intron length polymorphism
molecular markers
transferability
genetic diversity
 
Description Introns are noncoding sequences in a gene that are transcribed to precursor mRNA but spliced out during mRNA
maturation and are abundant in eukaryotic genomes. The availability of codominant molecular markers and saturated genetic
linkage maps have been limited in foxtail millet (Setaria italica (L.) P. Beauv.). Here, we describe the development of 98
novel intron length polymorphic (ILP) markers in foxtail millet using sequence information of the model plant rice. A total
of 575 nonredundant expressed sequence tag (EST) sequences were obtained, of which 327 and 248 unique sequences were
from dehydration- and salinity-stressed suppression subtractive hybridization libraries, respectively. The BLAST analysis of
98 EST sequences suggests a nearly defined function for about 64% of them, and they were grouped into 11 different functional categories. All 98 ILP primer pairs showed a high level of cross-species amplification in two millets and two nonmil-
lets species ranging from 90% to 100%, with a mean of ∼97%. The mean observed heterozygosity and Nei’s average gene
diversity 0.016 and 0.171, respectively, established the efficiency of the ILP markers for distinguishing the foxtail millet accessions. Based on 26 ILP markers, a reasonable dendrogram of 45 foxtail millet accessions was constructed, demonstrating
the utility of ILP markers in germplasm characterizations and genomic relationships in millets and nonmillets species.
Department of Science & Technology (DST),
Government of India for providing a DST-Young Scientist
fellowship to S.G. (SR/FT/LS-152/2008); and to NIPGR for
providing financial support
 
Date 2014-04-28T05:19:45Z
2014-04-28T05:19:45Z
2011
21 February 2011
 
Type Article
 
Identifier Genome, 54(7): 586-602
http://hdl.handle.net/123456789/189
 
Language en
 
Publisher NRC Res Press