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Overexpression of an apoplastic peroxidase gene CrPrx in transgenic hairy root lines of Catharanthus roseus

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Title Overexpression of an apoplastic peroxidase gene CrPrx in transgenic hairy root lines of Catharanthus roseus
 
Creator Jaggi, Monika
Kumar, Santosh
Sinha, Alok Krishna
 
Subject Agrobacterium rhizogenes
Catharanthus roseus
Monoterpenoid indole alkaloids
Peroxidases
Transgenic hairy roots
 
Description Peroxidases are a family of isoenzymes found in all higher plants and are known to be involved in a broad range of physiological processes. However, very little information is available concerning their role in Catharanthus roseus. The present study describes the impact of both overexpression and suppression of a peroxidase gene, CrPrx in C. roseus transgenic hairy root lines. Real-time PCR analysis in 35S-CrPrx and CrPrx-RNAi transgenic lines indicated differential transcript profile for peroxidases as well as for genes and regulators involved in MIA (monoterpenoid indole alkaloid) pathway of C. roseus. Comparative analysis revealed that MIA pathway genes showing elevated levels of expression in 35S-CrPrx transgenic lines showed a significant reduction in their transcript level in CrPrx-RNAi transgenic lines. Metabolite analysis detected higher levels of ajmalicine and serpentine accumulation in overexpressed lines. It was observed that all overexpressed transgenic lines produced more amount of H(2)O(2). These results indicate a role of CrPrx gene in the regulation of MIA pathway genes and regulators, thus affecting the production of specific alkaloids.
The work is supported by
financial assistance from the core grant of National Institute of Plant
Genome Research, New Delhi, India.
 
Date 2014-04-28T05:50:27Z
2014-04-28T05:50:27Z
2011
18 January 2011
 
Type Article
 
Identifier Applied Microbiology and Biotechnology, 90(3): 1005-1016
http://hdl.handle.net/123456789/192
 
Language en
 
Publisher Springer Science