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Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars [Setaria italica (L.)]

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Title Differential antioxidative responses to dehydration-induced oxidative stress in core set of foxtail millet cultivars [Setaria italica (L.)]
 
Creator Lata, Charu
Jha, Sarita
Dixit, Vivek
Sreenivasulu, Nese
Prasad, Manoj
 
Subject Setaria italica
Dehydration
Relative water content (RWC)
Lipid peroxidation (LP)
Electrolytic leakage (EL)
Reactive oxygen species (ROS)
Antioxidative enzymes
Quantitative real-time PCR (qRT-PCR)
 
Description Foxtail millet (Setaria italica L.) known as a relatively drought-tolerant crop across the world is grown in arid and semi-arid regions. To the best of our knowledge, no systematic study on drought tolerance screening of foxtail millet germplasm being a drought-tolerant crop has been reported so far. To explore genetic diversity of drought-induced oxidative stress tolerance in foxtail millet, we employed lipid peroxidation measure to assess membrane integrity under stress as biochemical marker to screen 107 cultivars and classified the genotypes as highly tolerant, tolerant, sensitive, and highly sensitive. From this comprehensive screening, four cultivars showing differential response to dehydration tolerance were selected to understand the physiological and biochemical basis of tolerance mechanisms. The dehydration-tolerant cultivars (IC-403579 and Prasad) showed considerably lower levels of lipid peroxidation and electrolyte leakage as compared with dehydration-sensitive cultivars (IC-480117 and Lepakshi), indicating better cell membrane integrity in tolerant cultivars. Correspondingly, tolerant genotypes maintained higher activity of catalase (EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and glutathione reductase (GR; EC 1.6.4.2) across different time-course period of polyethylene glycol (PEG) treatments in comparison to sensitive ones. The above biochemical results were further validated through quantitative real-time PCR analysis of APX and GR, whose transcripts were substantially induced by PEG treatments in tolerant cultivars. These results suggest that tolerant cultivars possess wider array of antioxidant machinery with efficient ascorbate-glutathione pathway to cope with drought-induced oxidative stress.
We are grateful to the Director, National Institute
of Plant Genome Research (NIPGR) for providing facilities and to
University Grants Commission (U.G.C.), Govt. of India for providing
UGC-SRF to Ms Charu Lata. We gratefully acknowledge the financial
support from the Department of Biotechnology, Government of India
(BT/IN/FRG/04/MP/2008), and IB-BMBF, Germany (IND 07/037), for
carrying out the present study under the Indo-German bilateral
program. We are also thankful to National Bureau of Plant Genetic
Resources, Hyderabad, India, for providing the seed material.
 
Date 2014-04-30T04:55:52Z
2014-04-30T04:55:52Z
2011
15 December 2010
 
Type Article
 
Identifier Protoplasma, 248(4): 817-828
http://hdl.handle.net/123456789/202
 
Language en
 
Publisher Springer Science