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Detection and mapping of quantitative trait loci for the contents of the terpenoid indole alkaloids, vindoline and catharanthine, in the leaves of Catharanthus roseus using bulk segregant analysis

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Title Detection and mapping of quantitative trait loci for the contents of the terpenoid indole alkaloids, vindoline and catharanthine, in the leaves of Catharanthus roseus using bulk segregant analysis
 
Creator Chaudhary, S
Pandey, R
Tripathi, B.N.
Goel, R
Kumar, S
 
Subject Catharanthus roseus
quantitative trait loci
terpenoid indole alkaloids
vindoline
catharanthine
segregant analysis
 
Description A mapping population consisting of 191 F2:7 recombinant inbred lines (RILs) of Catharanthus roseus was used for
segregation analysis of DNA markers, leading to the construction of a genetic linkage map of 174 DNA markers
placed on eight linkage groups (LG). These markers were present at an average inter-marker distance of 10.2 cM.
Leaves harvested from cultivated C. roseus plants are the main source of vindoline (V) and catharanthine (C), which
are chemically condensed during commercial synthesis of the widely-used anti-cancer drugs, vincristine and
vinblastine. To detect and map the major genes/quantitative trait loci (QTL) that control the accumulation of V and
C in C. roseus leaves, a bulk segregant analysis approach was used. The above mapping population of RILs was
phenotyped for their leaf alkaloid profiles and the distributions of V and C contents were used to assemble two pairs
of contrasting bulks of RILs, one each for V and C accumulation in extremely low or extremely high amounts. Primers
for the 174 already- mapped markers were PCR amplified on the bulks. Among the markers that discriminated
between the high-C and low-C bulks, and the high-V and low-V bulks, those that amplified all members of their
specific bulk were identified. This led to the detection of two QTLs (V1 and V2) and their co-segregating/linked DNA
markers for high-V content, and one QTL (C1) and its linked DNA markers for high-C content. These three QTLs
were then mapped. V1 and V2 were observed to lie on LG1, and C1 was on LG6. The QTLs V1 and V2 had large
additive effects. These, and C1, appear to be suitable for marker assisted selection.
We thank the Indian National Science Academy, the Council of Scientific and Industrial Research, and the Departments of Biotechnology and Science and Technology for financial support, and the Director of
NIPGR for access to facilities.
 
Date 2014-05-02T07:01:49Z
2014-05-02T07:01:49Z
2012
4 November 2011
 
Type Article
 
Identifier Journal of Horticultural Sci. and Biotechnol., 87(2): 179-185
http://hdl.handle.net/123456789/223
 
Language en
 
Publisher The Journal of Horticultural Science and Biotechnology