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Development of SSR markers and construction of a linkage map in jute

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Title Development of SSR markers and construction of a linkage map in jute
 
Creator Das, Moumita
Banerjee, Sumana
Dhariwal, Raman
Vyas, Shailendra
Mir, Reyazul R.
Topdar, Niladri
Kundu, Avijit
Khurana, Jitendra P.
Tyagi, Akhilesh K.
Sarkar, Debabrata
Sinha, Mohit K.
Balyan, Harindra S.
Gupta, PUSHPENDRA K.
 
Subject functional markers
genetic linkage map
recombinant inbred lines
SSR
SSR-enriched libraries
Corchorus olitorius
 
Description Jute is an important natural fibre crop, which is only second to cotton in its importance at the global level. It is mostly grown in
Indian subcontinent and has been recently used for the development of genomics resources. We recently initiated a programme
to develop simple sequence repeat markers and reported a set of 2469 SSR that were developed using four SSR-enriched
libraries (Mir et al. 2009). In this communication, we report an additional set of 607 novel SSR in 393 SSR containing
sequences. However, primers could be designed for only 417 potentially useful SSR. Polymorphism survey was carried out
for 374 primer pairs using two parental genotypes (JRO 524 and PPO4) of a mapping population developed for fibre fineness;
only 66 SSR were polymorphic. Owing to a low level of polymorphism between the parental genotypes and a high degree of
segregation distortion in recombinant inbred lines, genotypic data of only 53 polymorphic SSR on the mapping population
consisting of 120 RIL could be used for the construction of a linkage map; 36 SSR loci were mapped on six linkage groups
that covered a total genetic distance of 784.3 cM. Hopefully, this map will be enriched with more SSR loci in future and will
prove useful for identification of quantitative trait loci/genes for molecular breeding involving improvement of fibre fineness
and other related traits in jute.
 
Date 2014-05-05T10:30:55Z
2014-05-05T10:30:55Z
2012
February 2012
 
Type Article
 
Identifier J. Genet., 91: 21-31
http://hdl.handle.net/123456789/226
 
Language en
 
Publisher Indian Academy of Sciences