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Establishment of a photoautotrophic cell suspension culture of Arabidopsis thaliana for photosynthetic, metabolic, and signaling studies

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Title Establishment of a photoautotrophic cell suspension culture of Arabidopsis thaliana for photosynthetic, metabolic, and signaling studies
 
Creator Hampp, Christine
Richter, Andreas
Osorio, Sonia
Zellnig, Gunther
Sinha, Alok Krishna
Jammer, Alexandra
Fernie, Alisdair R.
Grimm, Bernhard
Roitsch, Thomas
 
Subject Photoautotrophic Cell
Arabidopsis thaliana
 
Description Plant cell suspension cultures have been used as model sys-
tems to circumvent the problems associated with the analyses
of a multi-factorial plant that is composed of multiple tissue
and cell types exposed to diverse signals. A number of plant
suspension cultures have proven to be valuable to study vari-
ous topics including defense response, secondary metabolite
formation, ion transport, gene regulation, and signal trans-
duction (Roitsch and Sinha, 2002 and references therein). How-
ever, most cultures reported to date, including the cultures
from model species such as Arabidopsis (Christie and Jenkins,
1996), require the presence of sugar in the medium and are
characterized by no or negligible photosynthetic activity
(Roitsch and Sinha, 2002). For only a very limited number of
species, such as Chenopodium rubrum, have true photoauto-
trophic (PA) cultures been established (see references in
Roitsch and Sinha, 2002). Such cultures combine the advan-
tages of plant suspension cultures with carbon autotrophy
and represent very powerful model systems for plant research.
Unfortunately, for as-yet unknown reasons, it is very difficult
to establish PA cultures (Widholm, 1992); hence, PA cultures
from only a few crop species have been established. PA cul-
tures have, however, been used to address various aspects
of photosynthesis, herbicide effects, and secondary product
formation from exclusively chloroplast localized pathways as
well as in characterization of the metabolic changes occurring
during the source–sink transition (Roitsch et al., 1995) and the
coordinated regulation of primary metabolism and defense
responses (Ehness et al., 1997). In parallel, a large number
of mutant and transgenic Arabidopsis plants have been char-
acterized with respect to their physiology, biochemistry, and
molecular biology. That said, the establishment of a PA cell
culture of Arabidopsis has, to date, proven elusive.
 
Date 2014-05-12T09:20:25Z
2014-05-12T09:20:25Z
2012
16 January 2012
 
Type Article
 
Identifier Mol. Plant, 5(2): 524-527
http://hdl.handle.net/123456789/235
 
Language en
 
Publisher Oxford University Press