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Differential transcript accumulation in chickpea during early phases of compatible interaction with a necrotrophic fungus Ascochyta rabiei

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Title Differential transcript accumulation in chickpea during early phases of compatible interaction with a necrotrophic fungus Ascochyta rabiei
 
Creator Jaiswal, Purnima
Cheruku, Jyothi Reddy
Kumar, Kamal
Yadav, Saurabh
Singh, Archana
Kumari, Pragati
Dube, Sunil Chandra
Upadhyaya, Kailash C.
Verma, Praveen K.
 
Subject Chickpea
Cicer arietinum
Suppression subtractive hybridization (SSH)
Ascochyta rabiei
Compatible interactions
Induced immunity
 
Description The initial phases of the disease establishment are very crucial for the compatible interactions. Pathogens must overcome the responses generated by the host for the onset of disease invasion. The compatible interaction is inadequately represented in plant-pathogen interaction studies. To gain broader insight into the early responses elicited by chickpea blight fungus Ascochyta rabiei during compatible interaction; we isolated early responsive genes of chickpea using PCR based suppression subtractive hybridization (SSH) strategy. We obtained ~250 unique genes after homology search and redundancy elimination. Based on their potential cellular functions, these genes were broadly classified into eleven different categories viz. stress, signaling, gene regulation, cellular metabolism and genes of unknown functions. Present study revealed few unexpected genes which have a possible role in induced immunity and disease progression. We employed macroarray, northern blot, real-time PCR and cluster analysis to develop transcript profiles. Most of the genes analyzed were early induced and were transcriptionally upregulated upon 24 h post inoculation. Our approach has rendered the isolation of early responsive genes involved in signaling and regulation of metabolic changes upon fungal infection. The information obtained will help to dissect the molecular mechanisms during compatible chickpea-Ascochyta interactions.
This work was supported by the research grant
provided by Department of Biotechnology, Government of India and
National Institute of Plant Genome Research, New Delhi.
 
Date 2014-05-13T11:13:44Z
2014-05-13T11:13:44Z
2012
14 September 2011
 
Type Article
 
Identifier Mol. Biol. Rep., 39(4): 4635-4646
http://hdl.handle.net/123456789/240
 
Language en
 
Publisher Springer Science