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In vitro and in vivo inhibition of Streptococcus mutans biofilm by Trachyspermum ammi seeds: an approach of alternative medicine

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Title In vitro and in vivo inhibition of Streptococcus mutans biofilm by Trachyspermum ammi seeds: an approach of alternative medicine
 
Creator Khan, Rosina
Adil, Mohd
Danishuddin, Mohd
Verma, Praveen K.
Khan, Asad U.
 
Subject Caries
Biofilm
Real time RT-PCR
Trachyspermum ammi
 
Description The aim of this study was to evaluate the influence of the crude and active solvent fraction of Trachyspermum ammi on S. mutans cariogenicity, effect on expression of genes involved in biofilm formation and caries development in rats. GC-MS was carried out to identify the major components present in the crude and the active fraction of T. ammi. The crude extract and the solvent fraction exhibiting least MIC were selected for further experiments. Scanning electron microscopy was carried out to observe the effect of the extracts on S. mutans biofilm. Comparative gene expression analysis was carried out for nine selected genes. 2-Isopropyl-5-methyl-phenol was found as major compound in crude and the active fraction. Binding site of this compound within the proteins involved in biofilm formation, was mapped with the help of docking studies. Real-time RT-PCR analyses revealed significant suppression of the genes involved in biofilm formation. All the test groups showed reduction in caries (smooth surface as well as sulcal surface caries) in rats. Moreover, it also provides new insight to understand the mechanism influencing biofilm formation in S. mutans. Furthermore, the data suggest the putative cariostatic properties of T. Ammi and hence can be used as an alternative medicine to prevent caries infection.
This work was supported by internal funds of Biotechnology
Unit, AMU and DST grant, 100/IFD/5160/2007-2008 to AUK.
 
Date 2014-05-15T11:37:59Z
2014-05-15T11:37:59Z
2012
May 2012
 
Type Article
 
Identifier Phytomedicine, 19(8-9): 747-755
http://hdl.handle.net/123456789/244
 
Language en
 
Publisher Elsevier