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Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae)

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Title Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae)
 
Creator Korekar, Girish
Sharma, Ram Kumar
Kumar, Rahul
Bisht, Naveen C.
Srivastava, Ravi B.
Ahuja, Paramvir Singh
Stobdan, Tsering
 
Subject Dioecious
Hippophae
RAPD
SCAR
Sex determination
 
Description The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened with 60 RAPD primers. Two primers, OPA-04 and OPT-06 consistently amplified female-specific (FS) polymorphic fragments of 1,164 and 868 bp, respectively, that were absent in the male samples. DNA sequence of the two markers did not exhibit significant similarity to previously characterized sequences. A sequence-characterized amplified region marker HrX1 (JQ284019) and HrX2 (JQ284020) designed for the two fragments, continued to amplify the FS allele in 120 female plants but not in 100 male plants tested in the current study. Thus, HrX1 and HrX2 are FS markers that can determine the sex of seabuckthorn plants in an early stage and expedite cultivations for industrial applications.
 
Date 2014-05-15T11:54:45Z
2014-05-15T11:54:45Z
2012
5 January 2012
 
Type Article
 
Identifier Biotechnol. Lett., 34(5): 973-978
http://hdl.handle.net/123456789/246
 
Language en
 
Publisher Springer Science