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Ectopic overexpression of vacuolar and apoplastic Catharanthus roseus peroxidases confers differential tolerance to salt and dehydration stress in transgenic tobacco

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Title Ectopic overexpression of vacuolar and apoplastic Catharanthus roseus peroxidases confers differential tolerance to salt and dehydration stress in transgenic tobacco
 
Creator Kumar, Santosh
Jaggi, Monika
Sinha, Alok Krishna
 
Subject Catharanthus roseus
Ectopic expression
Monoterpenoid indole alkaloid pathway
Overexpression
Oxidative stress
Peroxidase genes
 
Description CrPrx and CrPrx1 are class III peroxidases previously cloned and characterized from Catharanthus roseus. CrPrx is known to be apoplastic in nature, while CrPrx1 is targeted to vacuoles. In order to study their role in planta, these two peroxidases were expressed in Nicotiana tabacum. The transformed plants exhibited increased peroxidase activity. Increased oxidative stress tolerance was also observed in transgenics when treated with H(2)O(2) under strong light conditions. However, differential tolerance to salt and dehydration stress was observed during germination of T1 transgenic seeds. Under these stresses, the seed germination of CrPrx-transformed plants and wild-type plants was clearly suppressed, whereas CrPrx1 transgenic lines showed improved germination. CrPrx-transformed lines exhibited better cold tolerance than CrPrx1-transformed lines. These results indicate that vacuolar peroxidase plays an important role in salt and dehydration stress over cell wall-targeted peroxidase, while cell wall-targeted peroxidase renders cold stress tolerance.
This work is
supported by the core grant of the National Institute of Plant Genome
Research, New Delhi, India.
 
Date 2014-05-28T05:54:51Z
2014-05-28T05:54:51Z
2012
22 May 2011
 
Type Article
 
Identifier Protoplasma, 249(2): 423-432
http://hdl.handle.net/123456789/251
 
Language en
 
Publisher Springer Science