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Genetic dissection of grain weight in bread wheat through quantitative trait locus interval and association mapping

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Title Genetic dissection of grain weight in bread wheat through quantitative trait locus interval and association mapping
 
Creator Mir, RR
Kumar, N
Girdharwal, N
Prasad, Manoj
Balyan, HS
Gupta, PK
 
Subject Bread wheat
Grain weight
QTL interval mapping
Association mapping
 
Description Genetic dissection of grain weight in bread
wheat was undertaken through both genome-wide
quantitative trait locus (QTL) interval mapping and
association mapping. QTL interval mapping involved
preparation of a framework linkage map consisting of
294 loci {194 simple sequence repeats (SSRs), 86
amplified fragment length polymorphisms (AFLPs) and
14 selective amplifications of microsatellite polymor-
phic loci (SAMPL)} using a bi-parental recombinant
inbred line (RIL) mapping population derived from Rye
Selection111 9 Chinese Spring. Using the genotypic
data and phenotypic data on grain weight (GW) of RILs
collected over six environments, genome-wide single
locus QTL analysis was conducted to identify main
effect QTL. This led to identification of as many as ten
QTL including four major QTL (three QTL were
stable), each contributing [20% phenotypic variation
(PV) for GW. The above study was supplemented with
association mapping, which allowed identification of 11
new markers in the genomic regions that were not
reported earlier to harbour any QTL for GW. It also
allowed identification of closely linked markers for six
known QTL, and validation of eight QTL reported
earlier. The QTL identified through QTL interval
mapping and association mapping may prove useful in
marker-assisted selection (MAS) for the development of
cultivars with high GW in bread wheat.
Thanks are due to Department of
Biotechnology (DBT), Government of India for providing
financial support to carry out this study. Financial support was
also received from the DST through FIST-program and from the
University Grants Commission (UGC), New Delhi through
SAP-DRS program. R.R.M. was awarded Senior Research
Fellowships (SRF) by Council of Scientific & Industrial
Research (CSIR), New Delhi and P.K.G. was earlier awarded
a position of INSA Honorary Scientist and later a position of
NASI Senior Scientist.
 
Date 2015-10-27T08:34:39Z
2015-10-27T08:34:39Z
2012
 
Type Article
 
Identifier Mol. Breed., 29: 963-972
http://172.16.0.77:8080/jspui/handle/123456789/261
 
Language en_US
 
Publisher Springer