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The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet
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View Archive Info| Field | Value | |
| Title |
The DNA-binding activity of an AP2 protein is involved in transcriptional regulation of a stress-responsive gene, SiWD40, in foxtail millet
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| Creator |
Mishra, Awdhesh Kumar
Puranik, Swati Bahadur, Ranjit Prasad Prasad, Manoj |
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| Subject |
Abiotic stress
Protoplast co-transfection Dehydration-responsive element (DRE) Protein–protein docking Setaria italica WD40 protein Electrophoretic mobility shift assay (EMSA) |
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| Description |
A differentially expressed transcript, encoding a putative WD protein (Setaria italica WD40; SiWD40), was identified in foxtail millet. Tertiary structure modeling revealed that its C-terminus possesses eight blade β-propeller architecture. Its N-terminal has three α-helices and two 310-helices and was highly induced by different abiotic stresses. The SiWD40:GFP fusion protein was nuclear localized. Promoter analysis showed the presence of many cis-acting elements, including two dehydration responsive elements (DRE). A stress-responsive SiAP2 domain containing protein could specifically bind to these elements in the SiWD40 promoter. Thus, for the first time, we report that DREs probably regulate expression of SiWD40 during envi- ronmental stress. Molecular docking analysis revealed that the circumference of the β‐propeller structure was involved in an interaction with a SiCullin4 protein, supporting the adaptability of SiWD40 to act as a scaf- fold. Our study thus provides a vital clue for near future research on the stress-regulation of WD proteins. Grateful thanks are due to the Director, National Institute of Plant Genome Research (NIPGR), New Delhi, India for providing facilities. The authors work in this area was supported by NIPGR core-grant. We thank Dr. Supriya Chakraborty (Jawaharlal Nehru University, New Delhi, India) for kindly providing the BY-2 cell lines for our study and Dr. Swarup Parida, NIPGR, New Delhi for helpful discus- sions. Mr Awdhesh Kumar Mishra and Dr. Swati Puranik acknowledge the award of Research Fellowships from the Council of Scientific and Industrial Research, and NIPGR, New Delhi. We would like to thank all the reviewers for their constructive comments. |
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| Date |
2015-10-27T09:16:45Z
2015-10-27T09:16:45Z 2012 |
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| Type |
Article
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| Identifier |
Genomics, 100: 252-263
0888-7543 http://www.sciencedirect.com/science/article/pii/S0888754312001309 http://172.16.0.77:8080/jspui/handle/123456789/262 |
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| Language |
en_US
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| Publisher |
Elsevier
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