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Development of EST- derived SSR markers in pea (Pisum sativum) and their potential utility for genetic mapping and transferability

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Title Development of EST- derived SSR markers in pea (Pisum sativum) and their potential utility for genetic mapping and transferability
 
Creator Mishra, Raghvendra K
Gangadhar, Banieka H
Nookaraju, Akula
Kumar, Sushil
Park, Se W
 
Subject Pisum sativum
Medicago truncatula
expressed sequence tags
simple sequence repeats
polymorphism
microsatellites
 
Description Simple sequence repeats (SSRs) derived from expressed sequence tags (ESTs) are important resources for gene discovery and mapping. In this study, we developed EST-based SSR (eSSRs) markers and assessed their ability in mapping and transferability. A total of 10 800 unigenes were detected from 18 522 pea EST sequences (December 2009). Screening of 10 800 unigenes by MISA (MIcroSAtellite) revealed 2612 (14.1%) eSSRs in 2395 (12.9%) SSR-containing ESTs from which 577 (24.1%) primer pairs were designed. The most abundant repeat motif identified in eSSR was mononucleotide (85.2%), followed by trinucleotide (10.6%) and dinucleotide (2.8%). Among 108 randomly selected primer pairs, 40 were assessed for mapping and 68 to test cross-species transferability in six leguminous species. Out of 40 primer pairs, 85% produced amplicons, 60% showed polymorphism and 47.5% were mapped. Furthermore, 68 primer pairs revealed high rate of transferability (48–85%) in leguminous species. High levels of polymorphism, reproducibility, presence of alleles (3.8/locus) and transferability revealed the potential use of these eSSR markers in molecular mapping, quantitative trait loci (QTL) analysis and comparative mapping in pea and other legumes.
This paper was supported by Konkuk University in 2010. We thank
Dr. Manoj Prasad, NIPGR, New Delhi, India, for providing Medicago
EST-SSR sequences and Mr. Mayank and Ms. Sohyun for critical
reviewing of the manuscript. We would like to thank the reviewers for
their constructive comments.
 
Date 2015-10-27T09:44:18Z
2015-10-27T09:44:18Z
2012
 
Type Article
 
Identifier Plant Breeding, 131(1): 118-124
1439-0523
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2011.01926.x/abstract
http://172.16.0.77:8080/jspui/handle/123456789/264
 
Language en_US
 
Publisher Blackwell Verlag GmbH