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Analysis of differential expression of mediator subunit genes in Arabidopsis

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Title Analysis of differential expression of mediator subunit genes in Arabidopsis
 
Creator Pasrija, Richa
Thakur, Jitendra K.
 
Subject Mediator
Med subunit
hormones
stress
transcription
 
Description Mediator is a conserved eukaryotic multiprotein complex required by RNA polymerase II for transcription of its target genes. Till date, there is no report explaining the signals that affect the overall concentration of individual Med subunits. In this report, we have analyzed the effect of different phytohormones and stresses on the transcript level of Med genes in Arabidopsis. Hormones like auxin and JA, and cold stress did not show significant effect. ABA moderately increased the transcript abundance of more than 70% of AtMed genes analyzed in this study. However, there was noticeable change in the transcript level of several AtMed genes in response to BR. Stresses like high light, dark and salt also caused significant change in the transcript abundance of many AtMed genes. These data reveal that different environmental cues can affect stoichiometric concentration of Med subunits by affecting the transcription of their respective genes. This may, in turn, affect the overall arrangement of functional Mediator complex. This also suggests that some subunits may have some specific functions to play in response different signals.
This work was supported by IYBA grant (BT/BI/12/045/2008)
from Department of Biotechnology, Ministry of Science and
Technology, Government of India. We acknowledge the Central
Instrumentation Facility of NIPGR for real-time PCR reactions.
 
Date 2015-10-27T11:12:52Z
2015-10-27T11:12:52Z
2012
 
Type Article
 
Identifier Plant Signal. Behav., 7: 1676-1686
1559-2324 (Online)
http://www.tandfonline.com/doi/full/10.4161/psb.23983
http://172.16.0.77:8080/jspui/handle/123456789/267
 
Language en_US
 
Publisher Landes Bioscience