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HY1 genetically interacts with GBF1 and regulates the activity of the Z-box containing promoters in light signaling pathways in Arabidopsis thaliana

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Title HY1 genetically interacts with GBF1 and regulates the activity of the Z-box containing promoters in light signaling pathways in Arabidopsis thaliana
 
Creator Prasad, V. Babu Rajendra
Gupta, Nisha
Nandi, Ashis
Chattopadhyay, Sudip
 
Subject Photomorphogenesis
Gene expression
Arabidopsis
Seedling development
GBF1/ZBF2
HY1
 
Description Accepted date: 18 June 2012
Arabidopsis HY1/HO1, heme oxygenase enzyme, catalyses the oxygenation of heme to produce biliverdin, an essential step in the phytochrome-chromophore biosynthesis pathway. GBF1/ZBF2 is a G/Z-box binding bZIP protein that plays a dual but opposite regulatory roles in blue light-mediated seedling development and gene expression. Here, we show the genetic interactions of HY1 and GBF1 in seedling photomorphogenesis, and the role of HY1 in the regulation of promoters containing the Z-box light responsive element. Our results indicate that whereas the additional mutation in GBF1 does not affect the phenotype of hy1 mutant seedlings in red or far-red light, the additional mutation in HY1 suppresses the hyper photomorphogenic phenotype of gbf1 in BL. Further, transgenic studies using promoter-reporter constructs indicate that functional HY1 is essential for the optimal induction of Z-box containing synthetic and native promoters at various stages of Arabidopsis growth and development. Thus, this study establishes a functional relation of HY1 with GBF1, and HY1-mediated regulation of Z-box containing promoters in Arabidopsis seedling development.
This work is financially supported by Department of Biotechnology (DBT), Government of India to S.C and A.N.; N.G. is a Senior Research Fellow in the DBT funded project to S.C.
V.B.R.P is a recipient of CSIR/UGC fellowship, Government of India.
 
Date 2015-10-28T08:34:09Z
2015-10-28T08:34:09Z
2012
 
Type Article
 
Identifier Mechanisms of Development, 129: 298-307
0925-4773
http://www.sciencedirect.com/science/article/pii/S0925477312000573
http://172.16.0.77:8080/jspui/handle/123456789/270
 
Language en_US
 
Publisher Elsevier