Record Details

A physiologically regulated multidomain cystatin of wheat shows stage-dependent immunity against karnal bunt (Tilletia indica)

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title A physiologically regulated multidomain cystatin of wheat shows stage-dependent immunity against karnal bunt (Tilletia indica)
 
Creator Purwar, Shalini
Sundaram, Shanthy
Verma, Praveen K.
Srivastava, Shaili
Kumar, Anil
 
Subject Wheat
Tilletia indica
Phytocystatin
Antifungal protein
 
Description Accepted date: 5 October 2012
To identify novel components of basal resistance against the Tellitia indica of wheat, breeding for disease resistance was carried out on resistant and susceptible genotype of Karnal Bunt. The different members of wheat cystatin gene families were cloned, and their role in triggering differential resistance through co-expression was analyzed in our lab. The multidomain wheat cystatin (WCM) is a proteinase inhibitor characterized by cloning the gene from susceptible (WH542) and resistant genotype (HD 29). A WCM cDNA was isolated from both genotypes and sequenced. The WCM had a highly conserved N-terminal cystatin domain and a long C-terminal extension containing a second region, which exhibited similarity to the cystatin domain. The expression level was significantly (P > 0.001) higher in resistant compared to susceptible genotype at all the physiological stages of wheat spikes. In order to characterize the biochemical properties of WCM, the coding sequence was expressed in Escherichia coli using pET expression vector. The recombinant WCM was purified from soluble fraction of the cell extract by using affinity chromatography. WCM, with 23 KDa molecular mass, showed cysteine proteinase inhibitory activity against papain (Ki 3.039 × 10−7 M) as determined by using BAPNA as substrate. Furthermore, it was able to arrest the fungal mycelial growth of T. indica. Hyphae growth was inhibited, and morphological changes such as swelling and fragmentation of the fungus were observed. Overall, these observations suggest an endogenous high expression of cystatin, possibly associated with the resistance of wheat against Karnal bunt.
I am highly thankful to the Department of Science and Technology for the financial
support to complete this project. I am also thankful to Dr. Avinash Pandey, Coordinator, Nanotechnology
Application Center, Allahabad University, Allahabad for providing the Scanning Electron Microscope facility.
 
Date 2015-10-28T09:12:41Z
2015-10-28T09:12:41Z
2012
 
Type Article
 
Identifier Appl. Biochem. Biotechnol., 168(8): 2344-2357
1559-0291
http://link.springer.com/article/10.1007/s12010-012-9941-z
http://172.16.0.77:8080/jspui/handle/123456789/273
 
Language en_US
 
Publisher Springer