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Dynamics of defense related components in two contrasting genotypes to tomato upon infection with tomato leaf curl New Delhi virus

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Title Dynamics of defense related components in two contrasting genotypes to tomato upon infection with tomato leaf curl New Delhi virus
 
Creator Sahu, Pranav Pankaj
Rai, Neeraj Kumar
Puranik, Swati
Roy, Anirban
Khan, Moinuddin
Prasad, Manoj
 
Subject Antioxidant response
Gene expression
Quantitative real-time PCR
Short interfering RNA
Tomato
Tomato Leaf Curl New Delhi Virus
Virus resistance
 
Description Tomato leaf curl virus (ToLCV) disease is a serious threat for tomato cultivation in the tropics and subtropics. Despite serious efforts no immune commercial varieties or F(1) hybrids are available till date. In this study, the interaction between Solanum lycopersicum and ToLCV was characterized on molecular and biochemical basis. RNA silencing mediated by short interfering RNA (siRNA) and reactive oxygen species (ROS) has been proposed as central components of plant adaptation to several stresses. A comparative RNA interference study between two contrasting tomato genotypes, LA1777 (tolerant) and 15SBSB (susceptible) infected with Tomato Leaf Curl New Delhi Virus (ToLCNDV) revealed relatively higher accumulation of siRNA in the leaves of tolerant genotype. In LA1777, ToLCNDV produced chlorotic as well as necrotic areas at the inoculation sites 5-10 days post-inoculation. Caspase-9- and caspase-3-like activities were significantly increased in response to ToLCNDV infection in LA1777 at inoculated region. Activities of antioxidant enzymes involved in the detoxification of ROS were examined in both systemic and localized area of infection, and their expression level was further validated through quantitative real-time PCR of the corresponding transcripts. Expression patterns of three genes encoding pathogenesis-related proteins showed higher accumulation in tolerant genotype. Tolerance against the ToLCNDV in LA1777 can be attributed to the higher siRNA accumulation, localized cell death, altered levels of antioxidant enzymes and activation of pathogenesis-related genes at different durations of virus infection. Based on these direct and indirect evidences, we have proposed a putative mechanism for ToLCNDV tolerance in the tolerant genotype.
We are grateful to the Director, National
Institute of Plant Genome Research for providing facilities; to the
Council for Scientific and Industrial Research, Govt. of India for
providing a Senior Research Fellowship to Mr. Neeraj K Rai; to Dr.
S. Chakraborty, SLS, JNU, New Delhi, India, for providing virus
construct for agroinoculation and to Dr. A. T. Sadashiva, IIHR,
Bangalore, India, for seed material. We gratefully acknowledge the
financial support from the Department of Biotechnology, Government
of India (Grant no. BT/PR/5274/AGR/16/464/2004).
 
Date 2015-10-28T11:37:49Z
2015-10-28T11:37:49Z
2012
 
Type Article
 
Identifier Mol. Biotechnol., 52(2): 140-150
1559-0305
http://link.springer.com/article/10.1007%2Fs12033-011-9481-8
http://172.16.0.77:8080/jspui/handle/123456789/278
 
Language en_US
 
Publisher Springer