Record Details

COCHLEATA controls leaf size and secondary inflorescence architecture via negative regulation of UNIFOLIATA (LEAFY ortholog) gene in garden pea Pisum sativum

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title COCHLEATA controls leaf size and secondary inflorescence architecture via negative regulation of UNIFOLIATA (LEAFY ortholog) gene in garden pea Pisum sativum
 
Creator Sharma, Vishakha
Chaudhary, Swati
Kumar, Arvind
Kumar, Sushil
 
Subject Bracteole differentiation
COCHLEATA gene
garden pea
leaf differentiation
LEAFY ortholog
leaf size
Pisum sativum
secondary inflorescence
stub histology
UNIFOLIATA gene
UNIFOLIATA repression
UNIFOLIATA-TENDRILLED ACACIA gene
 
Description UNIFOLIATA [(UNI) or UNIFOLIATA-TENDRILLED ACACIA (UNI-TAC)] expression is known to be negatively regulated by COCHLEATA (COCH) in the differentiating stipules and flowers of Pisum sativum. In this study, additional roles of UNI and COCH in P. sativum were investigated. Comparative phenotyping revealed pleiotropic differences between COCH (UNI-TAC and uni-tac) and coch (UNI-TAC and uni-tac) genotypes of common genetic background. Secondary inflorescences were bracteole-less and bracteolated in COCH and coch genotypes, respectively. In comparison to the leaves and corresponding sub-organs and tissues produced on COCH plants, coch plants produced leaves of 1.5-fold higher biomass, 1.5-fold broader petioles and leaflets that were 1.8-fold larger in span and 1.2-fold dorso-ventrally thicker. coch leaflets possessed epidermal cells 1.3-fold larger in number and size, 1.4-fold larger spongy parenchyma cells and primary vascular bundles with 1.2-fold larger diameter. The transcript levels of UNI were at least 2-fold higher in coch leaves and secondary inflorescences than the corresponding COCH organs. It was concluded that COCH negatively regulated UNI in the differentiating leaves and secondary inflorescences and thereby controlled their sizes and/or structures. It was also surmised that COCH and UNI (LFY homolog) occur together widely in stipulate flowering plants.
We wish to acknowledge support of the Director, NIPGR,
in terms of provision of research facilities and help provided by Vinod Kumar in field crop management. The
research was funded by Indian National Science Academy
and Council of Scientific and Industrial Research (CSIR,
India) grants to SK and postgraduate fellowship grants
from CSIR to SC and SK and from SKA Institution for
Research, Education and Development to VS. We also
wish to thank Susan Singer for the highly useful discussion via email and Anand Sarkar and Bithika Sharma for
reading the manuscript.
 
Date 2015-10-29T07:19:45Z
2015-10-29T07:19:45Z
2012
 
Type Article
 
Identifier Journal of Biosciences, 37(6): 1041-1059
0973-7138
http://www.ias.ac.in/jbiosci/dec2012/1041
http://172.16.0.77:8080/jspui/handle/123456789/280
 
Language en_US
 
Publisher Indian Academy of Sciences