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Characterization of variation and quantitative trait loci related to terpenoid indole alkaloid yield in a recombinant inbred line mapping population of Catharanthus roseus

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Title Characterization of variation and quantitative trait loci related to terpenoid indole alkaloid yield in a recombinant inbred line mapping population of Catharanthus roseus
 
Creator Sharma, Vishakha
Chaudhary, Swati
Srivastava, Suchi
Pandey, Richa
Kumar, Sushil
 
Subject biomass
genetic map
harvest index
alkaloids
terpenoid indole alkaloid QTL
QTL map
 
Description Accepted date: 20 February 2012
Improved Catharanthus roseus cultivars are required for high yields of vinblastine, vindoline and catharanthine and/or serpen-
tine and ajmalicine, the pharmaceutical terpenoid indole alkaloids. An approach to derive them is to map QTL for terpenoid
indole alkaloids yields, identify DNA markers tightly linked to the QTL and apply marker assisted selection. Towards the
end, 197 recombinant inbred lines from a cross were grown over two seasons to characterize variability for seven biomass
and 23 terpenoid indole alkaloids content-traits and yield-traits. The recombinant inbred lines were genotyped for 178 DNA
markers which formed a framework genetic map of eight linkage groups (LG), spanning 1786.5 cM, with 10.0 cM average
intermarker distance. Estimates of correlations between traits allowed selection of seven relatively more important traits for
terpenoid indole alkaloids yields. QTL analysis was performed on them using single marker (regression) analysis, simple
interval mapping and composite interval mapping procedures. A total of 20 QTL were detected on five of eight LG, 10 for
five traits on LG1, five for four traits on LG2, three for one trait on LG3 and one each for different traits on LG three and
four. QTL for the same or different traits were found clustered on three LG. Co-location of two QTL for biomass traits was in
accord of correlation between them. The QTL were validated for use in marker assisted selection by the recombinant inbred
line which transgressively expressed 16 traits contributory to the yield vinblastine, vindoline and catharanthine from leaves
and roots that possessed favourable alleles of 13 relevant QTL.
The Director, NIPGR and Council of Scientific and Industrial
Research (CSIR) and Indian National Science Academy are gratefully thanked, respectively, for access to research facilities and
award of scientistship schemes to SK. CSIR and SKA Institution for
Research, Education and Development are thanked for the award of
postgraduate fellowships to SC and VS, respectively. Vinod Kumar
is thanked for his help in the field work.
 
Date 2015-10-29T08:22:14Z
2015-10-29T08:22:14Z
2012
 
Type Article
 
Identifier J. Genet., 91(1): 49-69
0973-7731
http://www.ias.ac.in/jgenet/Vol91No1/49.pdf
http://172.16.0.77:8080/jspui/handle/123456789/281
 
Language en_US
 
Publisher Indian Academy of Sciences