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Molecular interactions of GBF1 with HY5 and HYH during light-mediated seedling development in Arabidopsis thaliana

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Title Molecular interactions of GBF1 with HY5 and HYH during light-mediated seedling development in Arabidopsis thaliana
 
Creator Singh, Aparna
Ram, Hathi
Abbas, Nazia
Chattopadhyay, Sudip
 
Subject Development
Photomorphogenesis
Plant Molecular Biology
Transcription Factors
Transcription Regulation
HY5, HYH, ZBF2/GBF1
Gene Regulation
Photomorphogenesis
 
Description Accepted Date: June 2012
Arabidopsis bZIP transcription factor, GBF1, acts as a differential regulator of cryptochrome-mediated blue light signaling. Whereas the bZIP proteins, HY5 (elongated hypocotyl 5) and HYH (HY5 homologue), are degraded by COP1-mediated proteasomal pathways, GBF1 is degraded by a proteasomal pathway independent of COP1. In this study, we have investigated the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. The genetic studies using double and triple mutants reveal that GBF1 largely acts antagonistically with HY5 and HYH in Arabidopsis seedling development. Further, GBF1 and HY5 play more important roles than HYH in blue light-mediated photomorphogenic growth. This study reveals that GBF1 is able to form a G-box-binding heterodimer with HY5 but not with HYH. The in vitro and in vivo studies demonstrate that GBF1 co-localizes with HY5 or HYH in the nucleus and physically interacts with both of the proteins. The protein-protein interaction studies further reveal that the bZIP domain of GBF1 is essential and sufficient for the interaction with HY5 or HYH. Taken together, these data demonstrate the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development.
 
Date 2015-10-30T08:33:44Z
2015-10-30T08:33:44Z
2012
 
Type Article
 
Identifier J. Biol. Chem., 287(31): 25995-26009
1083-351X
http://www.jbc.org/content/287/31/25995.long
http://172.16.0.77:8080/jspui/handle/123456789/286
 
Language en_US
 
Publisher American Society for Biochemistry and Molecular Biology