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Comparative transcriptome analysis of the necrotrophic fungus Ascochyta rabiei during oxidative stress: insight for fungal survival in the host plant

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Title Comparative transcriptome analysis of the necrotrophic fungus Ascochyta rabiei during oxidative stress: insight for fungal survival in the host plant
 
Creator Singh, Kunal
Nizam, Shadab
Sinha, Manisha
Verma, Praveen K.
 
Description Accepted date: February 10, 2012
Localized cell death, known as the hypersensitive response (HR), is an important defense mechanism for neutralizing phytopathogens. The hallmark of the HR is an oxidative burst produced by the host plant. We aimed to identify genes of the necrotrophic chickpea blight fungus Ascochyta rabiei that are involved in counteracting oxidative stress. A subtractive cDNA library was constructed after menadione treatment, which resulted in the isolation of 128 unigenes. A reverse northern blot was used to compare transcript profiles after H2O2, menadione and sodium nitroprusside treatments. A total of 70 unigenes were found to be upregulated by more than two-fold following menadione treatment at different time intervals. A large number of genes not previously associated with oxidative stress were identified, along with many stress-responsive genes. Differential expression patterns of several genes were validated by quantitative real-time PCR (qRT-PCR) and northern blotting. In planta qRT-PCR of several selected genes also showed differential expression patterns during infection and disease progression. These data shed light on the molecular responses of the phytopathogen A. rabiei to overcome oxidative and nitrosative stresses and advance the understanding of necrotrophic fungal pathogen survival mechanisms.
This work was partially supported by a research grant from the Department of Biotechnology, Government of India (File No: BT/PR10605/PBD/16/791/
2008) [http://dbtindia.nic.in/dbt_new/appdproj2008-09.pdf]. The funders had no role in study design, data collection and analysis, decision to publish, or
preparation of the manuscript. No additional external funding received for this study.
 
Date 2015-10-30T08:45:48Z
2015-10-30T08:45:48Z
2012
 
Type Article
 
Identifier PLoS One, 7(3): e33128
1932-6203
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0033128
http://172.16.0.77:8080/jspui/handle/123456789/287
 
Language en_US
 
Publisher PLOS