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Comparative structural modelling of a monothiol GRX from chickpea: insight in iron-sulfur cluster assembly

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Title Comparative structural modelling of a monothiol GRX from chickpea: insight in iron-sulfur cluster assembly
 
Creator Yadav, Saurabh
Kushwaha, Hemant Ritturaj
Kumar, Kamal
Verma, Praveen K.
 
Subject Chickpea
GRX
Monothiol glutaredoxin
Homology modeling
Docking
Iron–sulfur cluster
 
Description Accepted date: 11 May 2012
Glutaredoxins (GRXs) are small, ubiquitous, multifunctional, heat-stable and glutathione-dependent thiol-disulphide oxidoreductases, classified under thioredoxin-fold superfamily. In the green lineage, GRXs constitute a complex family of proteins. Based on their active site, GRXs are classified into two subfamilies: dithiol and monothiol. Monothiol GRXs contain 'CGFS' as a redox active motif and assist in maintaining redox state and iron homeostasis within the cell. Using RACE strategy, a full length cDNA of chickpea (Cicer arietinum) glutaredoxin 3 (CarGRX3) was cloned and sequenced. The cDNA contains open reading frame of 537 bp encoding 178 amino acids and exhibits features of other known 'CGFS' type GRXs. Based on the multiple sequence alignment among CarGRX3 and monothiol GRXs of other photosynthetic organisms, the characteristic motif (KGX4PXCGFSX([29/30/32])KX4WPTXPQX4GX3GGXDI) with 18 invariant residues was observed. The proposed structure of CarGRX3 was compared with structurally resolved monothiol GRXs of other organisms. The CarGRX3 and nearest Arabidopsis homolog (AtGRXcp) shares 76% sequence identity which was reflected by their 3D-structure conservation. The structure of chickpea monothiol GRX (CarGRX3) coordinates glutathione ligated [2Fe-2S] cluster in a homodimeric form, highlighting the structural basis for iron-sulfur cluster (ISC) assembly and delivery to acceptor proteins. The present study on CarGRX3 model highlighted the utility of the theoretical approaches to understand complex biological phenomena such as glutathione docking and incorporation of GSH-ligated [2Fe-2S] cluster.
This work was supported by the research grant provided
by Department of Biotechnology, Government of India for Next
Generation Challenge Programme on Chickpea Genomics project
(Sanction No. BT/PR12919/AGR/02/676/2009) and National Institute of Plant Genome Research, New Delhi. S.Y. acknowledges University Grants Commission, India and K.K. to Council for Scientific and Industrial Research, India for Senior Research Fellowship.
 
Date 2015-10-30T10:07:11Z
2015-10-30T10:07:11Z
2012
 
Type Article
 
Identifier Int. J. Biol. Macromol., 51(3): 266-273
0141-8130
http://www.sciencedirect.com/science/article/pii/S0141813012001857
http://172.16.0.77:8080/jspui/handle/123456789/292
 
Language en_US
 
Publisher Elsevier