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Four genes encoding MYB28, a major transcriptional regulator of aliphatic glucosinolate pathway, are differentially expressed in the allopolyploid Brassica juncea

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Title Four genes encoding MYB28, a major transcriptional regulator of aliphatic glucosinolate pathway, are differentially expressed in the allopolyploid Brassica juncea
 
Creator Augustine, Rehna
Majee, Manoj
Gershenzon, Jonathan
Bisht, Naveen C.
 
Subject Brassica juncea
expression partitioning
glucosinolates
MYB28
transcription factor
 
Description Accepted date: 25 July 2013
Glucosinolates are Capparales-specific secondary metabolites that have immense potential in human health and agriculture. Unlike Arabidopsis thaliana, our knowledge about glucosinolate regulators in the Brassica crops is sparse. In the current study, four MYB28 homologues were identified (BjuMYB28-1,-2,-3,-4) from the polyploid Brassica juncea, and the effects of allopolyploidization on the divergence of gene sequence, structure, function, and expression were assessed. The deduced protein sequences of the four BjuMYB28 genes showed 76.1-83.1% identity with the Arabidopsis MYB28. Phylogenetic analysis revealed that the four BjuMYB28 proteins have evolved via the hybridization and duplication processes forming the B. juncea genome (AABB) from B. rapa (AA) and B. nigra (BB), while retaining high levels of sequence conservation. Mutant complementation and over-expression studies in A. thaliana showed that all four BjuMYB28 genes encode functional MYB28 proteins and resulted in similar aliphatic glucosinolate composition and content. Detailed expression analysis using qRT-PCR assays and promoter-GUS lines revealed that the BjuMYB28 genes have both tissue- and cell-specific expression partitioning in B. juncea. The two B-genome origin BjuMYB28 genes had more abundant transcripts during the early stages of plant development than the A-genome origin genes. However, with the onset of the reproductive phase, expression levels of all four BjuMYB28 increased significantly, which may be necessary for producing and maintaining high amounts of aliphatic glucosinolates during the later stages of plant development. Taken together, our results suggest that the four MYB28 genes are differentially expressed and regulated in B. juncea to play discrete though overlapping roles in controlling aliphatic glucosinolate biosynthesis.
We thank Dr Piero Moranidini for providing the homozygous myb28 knock-down line (BRC_H161b) and Dr Michael
Reichelt for his assistance with the glucosinolate analysis. We
thank Dr Arun Jagannath for his critical comments on the
manuscript. The central instrumentation and confocal facilities at NIPGR are acknowledged. RA was supported with
a Junior Research Fellowship from the Council of Scientific
and Industrial Research, India. This work was supported by
project schemes (BT/PR271/AGR/36/687/2011 and Rapid
Grant for Young Investigators [RGYI]) of the Department of
Biotechnology, India, and the core research grant was from
the National Institute of Plant Genome Research, India.
 
Date 2015-11-02T06:15:31Z
2015-11-02T06:15:31Z
2013
 
Type Article
 
Identifier J. Exp. Bot., 64(16): 4907-4921
0022-0957
http://jxb.oxfordjournals.org/content/64/16/4907.long#
http://172.16.0.77:8080/jspui/handle/123456789/294
 
Language en_US
 
Publisher Oxford University Press