Record Details

Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]
 
Creator Gupta, Sarika
Kumari, Kajal
Muthamilarasan, Mehanathan
Subramanian, Alagesan
Prasad, Manoj
 
Subject foxtail millet (Setaria italica L.)
microsatellite
SSRs
molecular markers
transferability
genetic diversity
 
Description Accepted date: March 16, 2013
Although the foxtail millet [Setaria italica (L.) P. Beauv.] is recently regarded as a model crop for studying functional genomics of biofuel grasses, its genetic improvement to some extent was limited due to the non-availability of molecular markers, particularly the microsatellite markers and the saturated genetic linkage map. Considering this, we attempted to generate a significant number of microsatellite markers in cultivar ‘Prasad’. Two hundred and fifty-six clones were sequenced to generate 41.82-kb high-quality sequences retrieved from genomic library enriched with dinucleotide repeat motifs. Microsatellites were identified in 194 (76%) of the 256 positive clones, and 64 primer pairs (pp) were successfully designed from 95 (49%) unique SSR-containing clones. The 67.4% primer designing ability, 100% PCR amplification efficiency and 45.3% polymorphic potential in the parents of F2 mapping population established the efficacy of genomic microsatellites. All the 64 microsatellite markers displayed high level of cross-species amplification (~67%) in 10 millets and non-millets species. These experimental findings suggest the utility and efficacy of SSRs in diverse genotyping applications, resolving QTLs, phylogenetic relationships and transferability in several important grass species.
We are grateful to the Director of National Institute of Plant Genome
Research (NIPGR) for providing facilities. Dr. Sarika Gupta acknowledges the award of DST-Young Scientist Fellowship (SR/FT/LS-152/2008) from the Department of Science & Technology (DST), Government of India, and Mr Mehanathan Muthamilarasan acknowledges the
award of Junior Research Fellowship from University Grants Commission, New Delhi. The study was supported by DST and NIPGR core
grant. We are thankful to NBPGR, New Delhi/Hyderabad/Akola, and
UAS, GKVK, Bangalore, India, for providing the seed materials.
 
Date 2015-11-04T09:00:49Z
2015-11-04T09:00:49Z
2013
 
Type Article
 
Identifier Plant Breeding, 132(4): 367-374
1439-0523
http://172.16.0.77:8080/jspui/handle/123456789/312
http://onlinelibrary.wiley.com/doi/10.1111/pbr.12070/abstract
10.1111/pbr.12070
 
Language en_US
 
Publisher John Wiley & Sons