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Promoter of CaZF, a chickpea gene that positively regulates growth and stress tolerance, is activated by an AP2-family transcription factor CAP2

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Title Promoter of CaZF, a chickpea gene that positively regulates growth and stress tolerance, is activated by an AP2-family transcription factor CAP2
 
Creator Jain, Deepti
Chattopadhyay, Debasis
 
Subject CaZF
Chickpea Gene
Stress Tolerance
AP2-Family
Transcription Factor
CAP2
 
Description Accepted date: January 13, 2013
Plants respond to different forms of stresses by inducing transcription of a common and distinct set of genes by concerted actions of a cascade of transcription regulators. We previously reported that a gene, CaZF encoding a C2H2-zinc finger family protein from chickpea (Cicer arietinum) imparted high salinity tolerance when expressed in tobacco plants. We report here that in addition to promoting tolerance against dehydration, salinity and high temperature, the CaZF overexpressing plants exhibited similar phenotype of growth and development like the plants overexpressing CAP2, encoding an AP2-family transcription factor from chickpea. To investigate any relationship between these two genes, we performed gene expression analysis in the overexpressing plants, promoter-reporter analysis and chromatin immunoprecipitation. A number of transcripts that exhibited enhanced accumulation upon expression of CAP2 or CaZF in tobacco plants were found common. Transient expression of CAP2 in chickpea leaves resulted in increased accumulation of CaZF transcript. Gel mobility shift and transient promoter-reporter assays suggested that CAP2 activates CaZF promoter by interacting with C-repeat elements (CRTs) in CaZF promoter. Chromatin immunoprecipitation (ChIP) assay demonstrated an in vivo interaction of CAP2 protein with CaZF promoter.
The project has been funded by National Institute of Plant Genome Research, New Delhi, India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-11-04T09:21:50Z
2015-11-04T09:21:50Z
2013
 
Type Article
 
Identifier PLoS One, 8(2): e56737
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/314
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0056737
10.1371/journal.pone.0056737
 
Language en_US
 
Publisher PLOS