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Comparative proteomics of dehydration response in the rice nucleus: new insights into the molecular basis of genotype-specific adaptation

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Title Comparative proteomics of dehydration response in the rice nucleus: new insights into the molecular basis of genotype-specific adaptation
 
Creator Jaiswal, Dinesh Kumar
Ray, Doel
Choudhary, Mani Kant
Subba, Pratigya
Kumar, Amit
Verma, Jitendra
Kumar, Rajiv
Datta, Asis
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Comparative proteomics
Dehydration response
Genotype-specific adaptation
Interactome
Nuclear fraction
Plant proteomics
 
Description Accepted date: September 23, 2013
Dehydration is the most crucial environmental factor that considerably reduces the crop harvest index, and thus has become a concern for global agriculture. To better understand the role of nuclear proteins in water-deficit condition, a nuclear proteome was developed from a dehydration-sensitive rice cultivar IR-64 followed by its comparison with that of a dehydration-tolerant c.v. Rasi. The 2DE protein profiling of c.v. IR-64 coupled with MS/MS analysis led to the identification of 93 dehydration-responsive proteins (DRPs). Among those identified proteins, 78 were predicted to be destined to the nucleus, accounting for more than 80% of the dataset. While the detected number of protein spots in c.v. IR-64 was higher when compared with that of Rasi, the number of DRPs was found to be less. Fifty-seven percent of the DRPs were found to be common to both sensitive and tolerant cultivars, indicating significant differences between the two nuclear proteomes. Further, we constructed a functional association network of the DRPs of c.v. IR-64, which suggests that a significant number of the proteins are capable of interacting with each other. The combination of nuclear proteome and interactome analyses would elucidate stress-responsive signaling and the molecular basis of dehydration tolerance in plants.
This work was supported by grants from the Council of Scientific and Industrial Research (CSIR), and the Department of
Biotechnology (DBT), Govt. of India. The authors thank the DBT
and CSIR for providing research fellowship to DKJ, DR, RK, and
MKC, PS. The authors also thank Dr. Vandenbrouck Yves for
helpful discussions and critical reading of the manuscript and
Jasbeer Singh for the technical assistance.
 
Date 2015-11-04T11:40:06Z
2015-11-04T11:40:06Z
2013
 
Type Article
 
Identifier Proteomics, 13(23-24): 3478-3497
1615-9861
http://172.16.0.77:8080/jspui/handle/123456789/317
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201300284/abstract
10.1002/pmic.201300284
 
Language en_US
 
Publisher John Wiley & Sons