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Spatial and temporal activity of upstream regulatory regions of rice anther-specific genes in transgenic rice and Arabidopsis

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Title Spatial and temporal activity of upstream regulatory regions of rice anther-specific genes in transgenic rice and Arabidopsis
 
Creator Khurana, Reema
Kapoor, Sanjay
Tyagi, Akhilesh K.
 
Subject Anther
Arabidopsis
GUS
GFP
Rice
Upstream regulatory region
 
Description Accepted date: 9 May 2012
Upstream regulatory regions (URRs) of rice anther-specific genes, namely OSbHLH (coding for basic helix-loop-helix-containing protein) and OSFbox (F-box protein encoding gene), selected from the microarray data have been cloned to control expression of GUS and GFP reporter genes in stably transformed rice. Quantitative real time PCR analysis shows maximum transcript accumulation of these two genes in the meiotic anthers. Analysis of OSbHLH and OSFbox URRs by PLACE database reveal the presence of known pollen-specific cis elements. The URRs of both OSbHLH and OSFbox genes have maximum activity in the meiotic anther stage in rice, but confer constitutive expression in the heterologous dicot system, Arabidopsis, indicative of monocot specificity. Another rice gene (OSIPK; with homology to genes encoding calcium-dependent protein kinases) URR already reported to have anther-specific activity in Arabidopsis and tobacco also confers anther-specific expression in rice and is active in the pollen tubes, suggesting it belongs to the category of late expressed genes. The spatial activity of three URRs has also been analysed by histochemical evaluation of GUS activity in different anther cells/tissues. The activity of OSIPK URR in rice is strongest among the three URRs.
This work was supported by the Department of Biotechnology and the Department of Science and
Technology, Government of India. RK is a recipient of JRF and
SRF from the Council of Scientific and Industrial Research.
 
Date 2015-11-05T05:37:09Z
2015-11-05T05:37:09Z
2013
 
Type Article
 
Identifier Transgenic Res., 22(1): 31-46
1573-9368
http://172.16.0.77:8080/jspui/handle/123456789/320
http://link.springer.com/article/10.1007%2Fs11248-012-9621-3
10.1007/s11248-012-9621-3
 
Language en_US
 
Publisher Springer