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A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis

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Title A 286 bp upstream regulatory region of a rice anther-specific gene, OSIPP3, confers pollen-specific expression in Arabidopsis
 
Creator Khurana, Reema
Kathuria, Hitesh
Mukhopadhyay, Arnab
Kapoor, Sanjay
Tyagi, Akhilesh K.
 
Subject Arabidopsis
GUS
Pollen
Rice
Upstream regulatory region
 
Description Accepted date: 12 November 2012
OSIPP3 gene (coding for pectin methylesterase inhibitor protein) was isolated from a pre-pollinated inflorescence-specific cDNA library by differential screening of stage-specific libraries from Oryza sativa. OSIPP3 is present in the genome of rice as a single copy gene. OSIPP3 gene was expressed exclusively in the pre-pollinated spikelets of rice. Upstream regulatory region (URR) of OSIPP3 was isolated and a series of 5'-deletions were cloned upstream of GUS reporter gene and were used to transform Arabidopsis. OSIPP3_del1 and del2 transgenic plants showed GUS expression in root, anther and silique, while OSIPP3_del3 showed GUS activity only in anthers and siliques. Pollen-specific expression was observed in case of plants harboring OSIPP3_del4 construct. It can, therefore, be concluded that the OSIPP3 URR between -178 and +108 bp is necessary for conferring pollen-specific expression in Arabidopsis.
This work was supported by the
Department of Biotechnology and the Department of Science
and Technology, Government of India. RK is a recipient of JRF
and SRF from the Council of Scientific & Industrial Research.
 
Date 2015-11-05T05:45:51Z
2015-11-05T05:45:51Z
2013
 
Type Article
 
Identifier Biotechnol. Lett., 35(3): 455-462
1573-6776
http://172.16.0.77:8080/jspui/handle/123456789/321
http://link.springer.com/article/10.1007%2Fs10529-012-1100-7
10.1007/s10529-012-1100-7
 
Language en_US
 
Publisher Springer