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Pleiotropic phenotypes of the salt-tolerant and cytosine hypomethylated leafless inflorescence, evergreen dwarf and irregular leaf lamina mutants of Catharanthus roseus possessing Mendelian inheritance

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Title Pleiotropic phenotypes of the salt-tolerant and cytosine hypomethylated leafless inflorescence, evergreen dwarf and irregular leaf lamina mutants of Catharanthus roseus possessing Mendelian inheritance
 
Creator Kumari, Renu
Sharma, Vishakha
Sharma, Vinay
Kumar, Sushil
 
Subject centromeric DNA
DNA hypomethylation
drought tolerance
epigenetic regulation
gene expression changes
microRNA
plant development
ribosomal DNA
salinity tolerance
terpenoid indole alkaloids
 
Description Accepted date: 23 May 2013
In Catharanthus roseus, three morphological cum salt-tolerant chemically induced mutants of Mendelian inheritance and their wild-type parent cv Nirmal were characterized for overall cytosine methylation at DNA repeats, expression of 119 protein coding and seven miRNA-coding genes and 50 quantitative traits. The mutants, named after their principal morphological feature(s), were leafless inflorescence (lli), evergreen dwarf (egd) and irregular leaf lamina (ill). The Southern-blot analysis of MspI digested DNAs of mutants probed with centromeric and 5S and 18S rDNA probes indicated that, in comparison to wild type, the mutants were extensively demethylated at cytosine sites. Among the 126 genes investigated for transcriptional expression, 85 were upregulated and 41 were downregulated in mutants. All of the five genes known to be stress responsive had increased expression in mutants. Several miRNA genes showed either increased or decreased expression in mutants. The C. roseus counterparts of CMT3, DRM2 and RDR2 were downregulated in mutants. Among the cell, organ and plant size, photosynthesis and metabolism related traits studied, 28 traits were similarly affected in mutants as compared to wild type. Each of the mutants also expressed some traits distinctively. The egd mutant possessed superior photosynthesis and water retention abilities. Biomass was hyperaccumulated in roots, stems, leaves and seeds of the lli mutant. The ill mutant was richest in the pharmaceutical alkaloids catharanthine, vindoline, vincristine and vinblastine. The nature of mutations, origins of mutant phenotypes and evolutionary importance of these mutants are discussed.
Grateful thanks are due to the Indian National Science Academy,
the Council of Scientific and Industrial Research, Government of India, and the Department of Biotechnology (DBT), Government
of India, for the grant of scientistship and research grant schemes,
respectively, to SK, to the Director NIPGR for grant of facilities and
to DBT and SKA Institution for Research, Education and Development for the grant of postgraduate fellowships to RK and VS,
respectively. Thanks are due to SK Raina for kindly sharing with us
the plasmid carrying 5 S rDNA of Lupin.
 
Date 2015-11-06T09:44:23Z
2015-11-06T09:44:23Z
2013
 
Type Article
 
Identifier J. Genet., 92(3): 369-394
0022-1333
http://172.16.0.77:8080/jspui/handle/123456789/337
http://www.ias.ac.in/describe/article/jgen/092/03/0369-0394
 
Language en_US
 
Publisher Indian Academy of Sciences